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首页> 外文期刊>Food Chemistry >Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics
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Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics

机译：利用近红外光谱和化学计量学对灵芝和灵芝中的总多糖和三萜类化合物进行定量

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摘要

Two chemometrics, the partial least-squares (PLS) and radial basis function (RBF) network were performed to develop a quantification method for total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum from different origins based on near infrared reflectance spectroscopy (NIR). The influences of spectral window and spectral pre-treatments were initially studied in the construction of PLS model. The best result was obtained when the standard normal transformation (SNV) +lst derivative spectrum over 4100-7750 cm~(-1) was used for the modelling. Then based on each principle, both of the two models were optimised respectively. The final results with high determination coefficient (R~2) (higher than 0.973, 0.989 for PLS and RBF, respectively) and low root mean square errors of prediction (RMSEP) (low to 0.1109 and 0.01298 for polysaccharides and triterpenoids, respectively) confirm the good predictability of the two models. The overall results show that NIR spectroscopy combined with chemometrics can be efficiently utilised for accurate analysis of routine chemical compositions in G. lucidum and G. atrum.
机译：基于近红外反射光谱法（NIR），进行了两种化学计量学的偏最小二乘（PLS）和径向基函数（RBF）网络，以开发灵芝和灵芝中不同来源的总多糖和三萜类化合物的定量方法。在建立PLS模型时，首先研究了光谱窗口和光谱预处理的影响。当使用标准正变换（SNV）+ 4100-7750 cm〜（-1）上的一阶导数谱进行建模时，可获得最佳结果。然后根据每个原理，分别对两个模型进行了优化。最终结果具有较高的测定系数（R〜2）（PLS和RBF分别高于0.973、0.989）和较低的预测均方根误差（RMSEP）（多糖和三萜类化合物分别低至0.1109和0.01298）两种模型的良好可预测性。总体结果表明，近红外光谱与化学计量学相结合可以有效地用于灵芝和灵芝中常规化学成分的准确分析。

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  • 来源
    《Food Chemistry》 |2012年第1期|p.268-275|共8页
  • 作者

    Yi Chen; Mingyong Xie; Hui Zhang; Yuanxing Wang; Shaoping Nie; Chang Li;

  • 作者单位

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    ganoderma lucidum; ganoderma atrum; near infrared spectroscopy; polysaccharides; triterpenoids; multivariate calibration;

    机译：灵芝灵芝近红外光谱多糖;三萜多元校正;

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