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首页> 外文期刊>Food microbiology >Modified atmosphere packaging decreased Pseudomonas fragi cell metabolism and extracellular proteolytic activities on meat
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Modified atmosphere packaging decreased Pseudomonas fragi cell metabolism and extracellular proteolytic activities on meat

机译：气调包装降低了对肉类的绿假单胞菌细胞代谢和细胞外蛋白水解活性

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摘要

Modified atmosphere packaging (MAP) is considered an effective method for extending the shelf life of meat. The use of optimal mixture of gases (CO2 and N-2) in food packaging containers has been proved to effectively inhibit the growth of microorganisms in poultry meat. In general, a minimum CO2 concentration range of 20%-30% is required for the inhibitory effect. The aim of this study was to investigate the mechanism by which MAP (CO2/N-2 30%/70%) inhibits Pseudomonas fragi, a dominant spoilage microorganism in aerobically stored chilled meat. The cell physiological changes were determined by measuring membrane integrity, membrane potential, ATP level, and extracellular proteolytic activity. The results showed that samples stored under MA retained cell membrane integrity, but lost significant membrane potential and ATP synthesis activity. Furthermore, the peptides issued from 2 structural proteins (myosin and actin) were mainly identified in air samples, indicating that these fragments result from bacterial proteolytic activity while MAP inhibited this activity. Overall, the study found that cell metabolism and extracellular protease activity decreased under MAP conditions. This study showed that MAP is an effective food preservation strategy and revealed mechanisms by which MAP inhibits spoilage.
机译：气调包装（MAP）被认为是延长肉类货架期的有效方法。已经证明在食品包装容器中使用最佳混合气体（CO2和N-2）可以有效抑制禽肉中微生物的生长。通常，抑制作用需要的最小CO2浓度范围为20％-30％。这项研究的目的是研究MAP（CO2 / N-2 30％/ 70％）抑制脆弱假单胞菌（Pseudomonas fragi）的作用机理。通过测量膜完整性，膜电位，ATP水平和细胞外蛋白水解活性来确定细胞的生理变化。结果表明，在MA下保存的样品保留了细胞膜的完整性，但失去了明显的膜电位和ATP合成活性。此外，主要从空气样品中鉴定出由2种结构蛋白（肌球蛋白和肌动蛋白）发出的肽，这表明这些片段是由细菌蛋白水解活性产生的，而MAP则抑制了这种活性。总体而言，该研究发现在MAP条件下细胞代谢和细胞外蛋白酶活性降低。这项研究表明，MAP是一种有效的食品保存策略，并揭示了MAP抑制腐败的机制。

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