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首页> 外文期刊>Food microbiology >Construction of stable fluorescent laboratory control strains for several food safety relevant Enterobacteriaceae
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Construction of stable fluorescent laboratory control strains for several food safety relevant Enterobacteriaceae

机译：几种与食品安全有关的肠杆菌科细菌的稳定荧光实验室控制菌株的构建

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Using naturally-occurring bacterial strains as positive controls in testing protocols is typically feared due to the risk of cross-contaminating samples. We have developed a collection of strains which express Green Fluorescent Protein (GFP) at high-level, permitting rapid screening of the following species on selective or non-selective plates: Escherichia coli O157:H7, Shigella sonnei, S. flexneri, Salmonella enterica subsp. Enterica serovar Gaminera, S. Mbandaka, S. Tennesse, S. Minnesota, S. Senftenberg and S. Typhimurium. These new strains fluoresce when irradiated with UV light and maintain this phenotype in absence of antibiotic selection. Recombinants were phenotypically equivalent to the parent strain, except for S. Tennessee Sal66 that appeared Lac(-) on Xylose Lysine Deoxycholate (XLD) agar plates and Lac thorn on Mac Conkey and Hektoen Enteric agar plates. Analysis of closed whole genome sequences revealed that Sal66 had lost one lactose operon; slower rates of lactose metabolism may affect lactose fermentation on XLD agar. These fluorescent enteric control strains were challenging to develop and should provide an easy and effective means of identifying cross-contamination. Published by Elsevier Ltd.
机译：由于存在交叉污染样品的风险，通常担心在测试方案中使用天然细菌菌株作为阳性对照。我们已经开发出了能高水平表达绿色荧光蛋白（GFP）的菌株，可以在选择性或非选择性平板上快速筛选以下物种：大肠杆菌O157：H7，志贺氏志贺氏菌，弗氏链球菌，肠沙门氏菌亚种Enterica serovar Gaminera，S。Mbandaka，S。Tennesse，S。Minnesota，S。Senftenberg和S. Typhimurium。这些新菌株在受到紫外线照射时发出荧光，并在没有选择抗生素的情况下保持这种表型。重组体在表型上与亲本菌株相同，除了在木糖赖氨酸脱氧胆酸盐（XLD）琼脂板上出现Lac（-），在Mac Conkey和Hektoen肠道琼脂板上出现Lac刺的S. Tennessee Sal66。对封闭的全基因组序列的分析表明，Sal66丢失了一个乳糖操纵子。较慢的乳糖代谢速率可能会影响XLD琼脂上的乳糖发酵。这些荧光肠控菌株难以开发，应提供一种简便有效的方法来鉴定交叉污染。由Elsevier Ltd.发布

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