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首页> 外文期刊>Food microbiology >Characterization of folate production and probiotic potential of Streptococcus gallolyticus subsp. macedonicus CRL415
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Characterization of folate production and probiotic potential of Streptococcus gallolyticus subsp. macedonicus CRL415

机译：溶链球菌亚种的叶酸产量和益生菌潜力的表征。马其顿CRL415

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Mandatory fortification of foods with folic acid is being questioned by many scientists principally because of the potential adverse secondary effects associated with their excessive consumption. It has been shown that selected strains of lactic acid bacteria (LAB) are able to produce natural forms of folate and these could be included in foods to prevent deficiencies without causing adverse effects. The aim of this study was to evaluate folate production and fol gene expression by Streptococcus gallolyticus subsp. macedonicus (S. macedonicus) CRL415 under different growth conditions in vitro and to assess its potential probiotic application. Results showed that glucose as the principal carbon source, and incubation at 42 degrees C under controlled pH conditions (6.0) increased folate production, fol gene expression, and growth of S. macedonicus CRL415. This strain was able to produce elevated folate concentrations during milk fermentation without the need of prolonged incubation times and was able to resist conditions simulating the gastrointestinal tract. In addition, S. macedonicus was susceptible to all required antibiotics, and had a good adhesion level to intestinal cells in vitro, making it a promising candidate for biotechnological application as functional starter cultures in the dairy industry.
机译：许多科学家对用叶酸强制性强化食品提出质疑，主要是因为食用过量叶酸可能会产生不利的次级影响。已经表明，选定的乳酸菌菌株（LAB）能够产生天然形式的叶酸，并且可以将它们包含在食品中以防止缺乏而不会造成不利影响。这项研究的目的是评估解链链球菌亚种的叶酸产量和fol基因表达。 Macedonicus（S. macedonicus）CRL415在不同的生长条件下体外进行，并评估其潜在的益生菌应用。结果表明，葡萄糖是主要的碳源，在42摄氏度的pH值受控条件下（6.0）孵育可提高马其顿酵母CRL415的叶酸产量，fol基因表达和生长。该菌株能够在牛奶发酵过程中产生较高的叶酸浓度，而无需延长孵育时间，并且能够抵抗模拟胃肠道的条件。此外，马其顿氏菌对所有必需的抗生素均敏感，并且在体外对肠道细胞具有良好的粘附水平，使其成为生物技术应用的有前途的候选者，成为乳制品工业中的功能发酵剂。

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