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首页> 外文期刊>Food microbiology >Prevalence and genetic characteristics of Cronobacter spp. from food and human clinical stool samples in Wenzhou, China 2008-2018
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Prevalence and genetic characteristics of Cronobacter spp. from food and human clinical stool samples in Wenzhou, China 2008-2018

机译：克氏杆菌属的流行和遗传特征。从中国温州的食物和人类临床粪便样本中提取2008-2018

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Pathogenic Cronobacter species are responsible for life-threatening illness in neonates. A ten-year comprehensive survey was conducted to examine the population structure and antimicrobial resistant patterns of Cronobacter isolates from food (n = 78) and clinical (n = 12) sources in Wenzhou, China. A total of 90 (4.4%) isolates were recovered from 2051 collected samples. The occurrence of Cronobacter spp. was highest in spices with a rate of 22% (26/119), whereas the lowest contamination rate of 1% was found in powered infant and toddler formula (7/494), special medical infant formula (1/95) and human stool samples (12/1024). Cronobacter strains revealed a high degree of genetic diversity among the isolates tested. Pulsed-field gel electrophoresis (PFGE) distinguished 75 clonal groups, and the biggest cluster consisted of four strains. Multilocus sequence typing (MLST) method displayed 43 sequence types (STs), of which ST1, ST4, ST8, ST64, ST148 and ST201 were most frequently identified. Meanwhile, two new sequence types were discovered and added to the PubMLST international database. Resistance to ceftriaxone, cefotaxiv, amoxicillin, ampicillin, cefoxitin, tetracycline, streptomycin, azithromycin, chloramphenicol, as well as multidrug resistance, was noted. Taken together, this large-scale surveillance study highlights the wide dissemination and diverse molecular features of Cronobacter spp. in Wenzhou China.
机译：致病性克罗诺杆菌病是造成新生儿生命危险的疾病。进行了为期十年的全面调查，以检查来自中国温州的食物（n = 78）和临床（n = 12）来源的克氏杆菌分离株的种群结构和抗微生物模式。从2051个收集的样品中总共回收了90个（4.4％）分离株。克氏杆菌属的发生。在调味品中含量最高，为22％（26/119），而在电动婴幼儿配方奶粉（7/494），特殊医用婴儿配方奶粉（1/95）和人类粪便中发现的最低污染率为1％样本（12/1024）。克罗诺杆菌菌株在测试的分离物中显示出高度的遗传多样性。脉冲场凝胶电泳（PFGE）区分了75个克隆组，最大的簇由四个菌株组成。多基因座序列分型（MLST）方法显示了43种序列类型（ST），其中最常识别出ST1，ST4，ST8，ST64，ST148和ST201。同时，发现了两种新的序列类型并将其添加到PubMLST国际数据库中。注意到对头孢曲松，头孢噻呋，阿莫西林，氨苄青霉素，头孢西丁，四环素，链霉素，阿奇霉素，氯霉素的耐药性，以及多药耐药性。总体而言，这项大规模的监测研究突显了Cronobacter spp的广泛传播和多样的分子特征。在中国温州。

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