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首页> 外文期刊>Food microbiology >Antiviral effects of blueberry proanthocyanidins against Aichi virus
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Antiviral effects of blueberry proanthocyanidins against Aichi virus

机译：蓝莓金属蛋白对AICHI病毒的抗病毒作用

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摘要

Blueberry polyphenols are known for their high antioxidant and antimicrobial potential. Aichi virus (AiV) is an emerging human enteric virus that causes gastroenteritis outbreaks worldwide. This study aimed to (1) determine the time-and dose-dependent effects of blueberry proanthocyanidins (B-PAC) against AiV over 24 h at 37 degrees C; (2) gain insights on their mode of action using pre- and post-treatment of host cells and Transmission Electron Microscopy; and (3) determine their anti-AiV effects in model foods and under simulated gastric conditions. AiV at similar to 5 log PFU/ml was incubated with equal volumes of commercial blueberry juice (BJ, pH 2.8), neutralized BJ (pH 7.0), B-PAC (2, 4, and 10 mg/ml) prepared either in 10% ethanol, apple juice (AJ), 2% milk, simulated gastric fluid (SGF, pH 1.5) or simulated intestinal fluid (SIF, pH 7.5), and controls (malic acid (pH 3.0), phosphate buffered saline (pH 7.2), apple juice (pH 3.6) and 2% milk) over 24 h at 37 degrees C, followed by standard plaque assays. Each experiment was replicated thrice and data were statistically analyzed. Differences in AiV titers with 1 mg/ml B-PAC were 2.13 +/- 0.06 log PFU/ml lower after 24 h and = 3 log PFU/ml (undetectable levels) lower with 2 and 5 mg/ml B-PAC compared to AiV titers in PBS after 24 h and 3 h, respectively. BJ at 37 degrees C resulted in titer differences (lower titers compared to PBS) of 0.17 +/- 0.06, 1.27 +/- 0.01, and 1.73 +/- 0.23 log PFU/ml after 1, 3, and 6 h and = 3 log PFU/ml after 24 h. Pre- and post-treatment of host cells with 0.5 mg/ml B-PAC caused titer decreases of 0.62 +/- 0.33 and 0.30 +/- 0.06 log PFU/ml, respectively suggesting a moderate effect on viral-host cell binding. B-PAC at 2 mg/ml in AJ caused titer differences of = 3 log PFU/ml after 0.5 h, while differences of 0.84 +/- 0.03 log PFU/ml with 5 mg/ml B-PAC in milk, and = 3 log PFU/ml with B-PAC at 5 mg/ml in SIF after 30 min were obtained. This study shows the ability of BJ and B-PAC to decrease AiV titers to potentially prevent AiV-related illness and outbreaks.
机译：以其高抗氧化剂和抗微生物潜力而闻名蓝莓多酚。 Aichi病毒（AIV）是一种新兴人肠道病毒，导致全世界胃肠炎爆发。本研究旨在（1）确定蓝莓花青蛋白（B-PAC）在37摄氏度上超过24小时AIV的时间和剂量依赖性作用; （2）使用宿主细胞和透射电子显微镜的预处理和后处理来获得对其作用方式的见解; （3）确定其模型食品中的抗AIV效应以及模拟胃条件。将AIV类似于5种LOG PFU / mL，与同等量的商用蓝莓汁（BJ，pH 2.8），中和的BJ（pH7.0），B-PAC（2,4和10mg / ml）一起孵育，其在10中％乙醇，苹果汁（AJ），2％牛奶，模拟胃液（SGF，pH 1.5）或模拟肠液（SIF，pH 7.5）和对照（苹果酸（pH 3.0），磷酸盐缓冲盐水（pH7.2） ，苹果汁（pH 3.6）和2％乳）在37℃下超过24小时，然后是标准斑块测定。每个实验都被复制三次，数据进行了统计分析。 24小时后，1mg / ml B-PAC的AIV滴度的差异为2.13 +/- 0.06 log pfu / ml，> = 3 log pfu / ml（未检测水平）低2和5mg / ml B-PAC比较分别在24小时和3小时后PBS中的AIV滴度。 37摄氏度的BJ导致滴度差异（与PBS相比的低滴度）为0.17 +/- 0.06,1.27 +/- 0.01和1.73 +/- 0.23 Log PFU / ml，在1,3和6小时后，> => = 3 24小时后3 log pfu / ml。具有0.5mg / ml B-PAC的宿主细胞的预处理和后处理导致滴度的滴度为0.62 +/- 0.33和0.30 +/- 0.06 Log Pfu / ml，分别表明对病毒宿主细胞结合的中度效应。在0.5小时后引起滴度差异> = 3 log pfu / ml的滴度差异，而在牛奶中为5mg / ml B-PAC的差异为0.84 +/- 0.03对数值/ ml，含有5mg / ml B-PAC的差异，差异为0.84 +/- 0.03对牛奶中的差异，> = 3在获得30分钟后，在SIF中，在5mg / ml下用B-PAC的LOG PFU / ml在获得30分钟后。本研究表明，BJ和B-PAC降低AIV滴度，以潜在地预防与AIV相关的疾病和爆发的能力。

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