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首页> 外文期刊>Food microbiology >Transcriptional profiling and metabolomic analysis of Staphylococcus aureus grown on autoclaved chicken breast
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Transcriptional profiling and metabolomic analysis of Staphylococcus aureus grown on autoclaved chicken breast

机译：在高压灭菌鸡胸肉上生长的金黄色葡萄球菌的转录分析和代谢组分分析

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摘要

Although Staphylococcus aureus is a major cause of food poisoning, little is known about its response to growth on food. Utilizing a transcriptional profiling and metabolomics approach, we compared S. aureus grown on autoclaved chicken breast (ACB) to Luria broth agar. ACB cultures demonstrated increased expression of genes associated with protein synthesis, cofactors, secondary metabolites, nitrogen and nucleotide metabolism, amino acid transport, and reduced expression of general stress, lipid metabolism, and virulence genes. The ACB culture also displayed characteristics of catabolite de-repression and anaerobic growth, and increased expression of arginine biosynthesis genes (argFGH) and an arginine/ornithine antiporter gene (arcD). S. aureus synthesizes arginine from proline and the ACB culture exhibited increased expression of proline transport genes (opuBA, opuBB and putP) and increased proline accumulation. Amino acid and sugar content in the ACB grown culture increased, and this was attributed to the consumption of ACB, transport of amino acids, and gluconeogenesis. Genes involved with biotin biosynthesis and uptake were upregulated and biotin is required for amino acid catabolism. Genes encoding urease and urease activity were upregulated in ACB cultures, while urea levels were reduced. This research provides fundamental information on the response of S. aureus growing on chicken meat that could find application in future attempts to reduce the growth of S. aureus in food.
机译：虽然金黄色葡萄球菌是食物中毒的主要原因，但对其对食物生长的反应很少。利用转录分析和代谢组种方法，我们将在高压灭菌的鸡胸肉（ACB）上生长的金黄色葡萄球菌进行比较至LURIA肉汤琼脂。 ACB培养物证明了与蛋白质合成，辅因子，次生代谢物，氮和核苷酸代谢，氨基酸输送的基因的表达增加，以及减少一般应激，脂质代谢和毒力基因的表达。 ACB培养也显示了分解粘土脱垢和厌氧生长的特征，并增加了精氨酸生物合成基因（ARGFGH）和精氨酸/鸟氨酸炔醇基因（ARCD）的表达。 S.金黄色葡萄球菌合成来自脯氨酸的精氨酸，ACB培养表现出增加脯氨酸输送基因（OPUBA，OPBB和PUTP）的表达，并增加脯氨酸积累。 ACB的氨基酸和糖含量增加，这归因于ACB的消耗，氨基酸的运输和葡糖生成。涉及生物素生物合成和摄取的基因是上调的，并且氨基酸分解代谢需要生物素。编码脲酶和脲酶活性的基因在ACB培养物中上调，而尿素水平降低。本研究提供了有关在鸡肉中生长的响应的基本信息，可以在未来试图降低食物中的S.金黄色葡萄球菌的生长中找到应用。

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  • 来源
    《Food microbiology》 |2019年第9期|46-52|共7页
  • 作者

    Dupre Joanne M.; Johnson William L.; Ulanov Alexander V; Li Zhong; Wilkinson Brian J.; Gustafson John E.;

  • 作者单位

    New Mexico State Univ Dept Biol POB 30001 MSC 3AF Las Cruces NM 88003 USA;

    Oklahoma State Univ Dept Biochem & Mol Biol 246 Noble Res Ctr Stillwater OK 74078 USA;

    Univ Illinois Roy J Carver Biotechnol Ctr 407 S Goodwin Champaign IL 61801 USA;

    Univ Illinois Roy J Carver Biotechnol Ctr 407 S Goodwin Champaign IL 61801 USA;

    Illinois State Univ Sch Biol Sci Normal IL 61790 USA;

    New Mexico State Univ Dept Biol POB 30001 MSC 3AF Las Cruces NM 88003 USA|Oklahoma State Univ Dept Biochem & Mol Biol 246 Noble Res Ctr Stillwater OK 74078 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    S. aureus; Chicken; Transcriptional profiling; Metabolomics;

    机译：S. aureus;鸡;转录分析;代谢组学;

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