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首页> 外文期刊>Food microbiology >Surveillance of norovirus contamination in commercial fresh/frozen berries from Heilongjiang Province, China, using a TaqMan real-time RT-PCR assay
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Surveillance of norovirus contamination in commercial fresh/frozen berries from Heilongjiang Province, China, using a TaqMan real-time RT-PCR assay

机译：使用Taqman实时RT-PCR测定，来自中国黑龙江省商业新鲜/冷冻浆果的诺罗维病毒污染监测

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摘要

Norovirus (NoV), a major food-borne virus, causes non-bacterial acute gastroenteritis in humans. Berries are generally harvested from low-growing bushes by hand and are minimally processed before being sold to consumers. Therefore, the consumption of berries has been linked to numerous food-borne gastroenteritis outbreaks caused by NoV in many countries. We performed a survey of NoV contamination in commercial fresh/frozen berry fruits collected from 2016 to 2017 in the Heilongjiang Province, the main berry-producing area in China, using a TaqMan-based real-time reverse transcription-PCR assay. Among 900 frozen and 900 fresh domestic retail berry samples, the prevalence of NoV was 9% (81/900) and 12.11% (109/900), including 35.80% (29/81) and 29.36% (32/109) of genotype GI alone, 54.32% (44/81) and 60.55% (66/109) of GII alone, and 9.88% (8/81) and 10.09% (11/109) of both GI and GII, respectively. No NoV was detected among the 677 frozen berry samples for export. Thus, the occurrence of NoV contamination was significantly higher in domestic berries than in exported berries and higher in fresh berries than in frozen berries. This study highlights the need for further risk surveillance for NoV contamination in berries produced in the Heilongjiang Province and recommends region-extended monitoring of retail berries for NoV.
机译：诺罗病毒（11月）是一种主要的食物传播的病毒，导致人类中的非细菌急性胃肠炎。浆果通常通过手工从低生长的灌木丛中收获，并且在销售给消费者之前是微量的处理。因此，浆果的消费与许多国家11月份造成的众多食物传播的胃肠炎爆发有关。我们对2016年至2017年2016年至2017年的商业新鲜/冷冻浆果水果中的11月污染调查，是在中国主要的浆果生产区域，利用基于Taqman的实时逆转录-PCR测定。在900个冷冻和900家新鲜的国内零售浆果样本中，11月的患病率为9％（81/900）和12.11％（109/900），其中包括35.80％（29/81）和29.36％（32/109）基因型单独的GI，54.32％（44/81）和60.55％（66/109）的GII，分别为9.88％（8/81）和10.09％（11/109），两种GI和GII。在677个冷冻浆果样品中没有检测到11月出口。因此，在国内浆果中的11月污染的发生显着高于出口的浆果，新鲜浆果高于冷冻浆果。本研究强调了对黑龙江省生产中生产的浆果中新污染的进一步风险监测，并建议延长11月零售浆果监测。

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  • 来源
    《Food microbiology》 |2019年第9期|119-126|共8页
  • 作者

    Gao Xuwen; Wang Ziwei; Wang Yixin; Liu Zhongmei; Guan Xueting; Ma Yingying; Zhou Han; Jiang Yanping; Cui Wen; Wang Li; Xu Yigang;

  • 作者单位

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Heilongjiang Entry Exit Inspect & Quarantine Bur Technol Ctr Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Anim Sci & Technol Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China|Heilongjiang Key Lab Anim Dis Control & Pharmaceu Harbin Heilongjiang Peoples R China;

    Northeast Agr Univ Coll Vet Med Changjiang Rd 600 Harbin Heilongjiang Peoples R China|Heilongjiang Key Lab Anim Dis Control & Pharmaceu Harbin Heilongjiang Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Norovirus; Berries; Real-time RT-PCR; Risk surveillances;

    机译：诺罗病毒;浆果;实时RT-PCR;风险监测;

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