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首页> 外文期刊>Food microbiology >Surveillance of norovirus contamination in commercial fresh/frozen berries from Heilongjiang Province, China, using a TaqMan real-time RT-PCR assay
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Surveillance of norovirus contamination in commercial fresh/frozen berries from Heilongjiang Province, China, using a TaqMan real-time RT-PCR assay

机译：使用TaqMan实时RT-PCR测定法对中国黑龙江省商业新鲜/冷冻浆果中的诺如病毒污染进行监测

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摘要

Norovirus (NoV), a major food-borne virus, causes non-bacterial acute gastroenteritis in humans. Berries are generally harvested from low-growing bushes by hand and are minimally processed before being sold to consumers. Therefore, the consumption of berries has been linked to numerous food-borne gastroenteritis outbreaks caused by NoV in many countries. We performed a survey of NoV contamination in commercial fresh/frozen berry fruits collected from 2016 to 2017 in the Heilongjiang Province, the main berry-producing area in China, using a TaqMan-based real-time reverse transcription-PCR assay. Among 900 frozen and 900 fresh domestic retail berry samples, the prevalence of NoV was 9% (81/900) and 12.11% (109/900), including 35.80% (29/81) and 29.36% (32/109) of genotype GI alone, 54.32% (44/81) and 60.55% (66/109) of GII alone, and 9.88% (8/81) and 10.09% (11/109) of both GI and GII, respectively. No NoV was detected among the 677 frozen berry samples for export. Thus, the occurrence of NoV contamination was significantly higher in domestic berries than in exported berries and higher in fresh berries than in frozen berries. This study highlights the need for further risk surveillance for NoV contamination in berries produced in the Heilongjiang Province and recommends region-extended monitoring of retail berries for NoV.
机译：诺如病毒（NoV）是一种主要的食源性病毒，可引起人类非细菌性急性胃肠炎。浆果通常是从低矮的灌木丛中手工收获的，在出售给消费者之前经过最少的加工。因此，浆果的消费与许多国家由新病毒引起的许多食源性胃肠炎暴发有关。我们使用基于TaqMan的实时逆转录PCR技术对2016年至2017年在中国主要浆果产区黑龙江省采集的商业新鲜/冷冻浆果进行了NoV污染调查。在900份冷冻和900份新鲜的国内零售浆果样品中，NoV的流行率为9％（81/900）和12.11％（109/900），包括基因型的35.80％（29/81）和29.36％（32/109）仅GI，分别占GII的54.32％（44/81）和60.55％（66/109），分别占GI和GII的9.88％（8/81）和10.09％（11/109）。在677个出口的冷冻浆果样品中未检测到NoV。因此，国内浆果中NoV污染的发生率显着高于出口浆果，而新鲜浆果中NoV污染的发生率高于冷冻浆果。这项研究强调需要对黑龙江省生产的浆果中的NoV污染进行进一步的风险监测，并建议对零售浆果进行NoV的区域扩展监测。

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  • 来源
    《Food microbiology》 |2019年第9期|119-126|共8页
  • 作者

    Gao Xuwen; Wang Ziwei; Wang Yixin; Liu Zhongmei; Guan Xueting; Ma Yingying; Zhou Han; Jiang Yanping; Cui Wen; Wang Li; Xu Yigang;

  • 作者单位

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Heilongjiang Entry Exit Inspect & Quarantine Bur, Technol Ctr, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Anim Sci & Technol, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China|Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Harbin, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Heilongjiang, Peoples R China|Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Harbin, Heilongjiang, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Norovirus; Berries; Real-time RT-PCR; Risk surveillances;

    机译：诺如病毒;浆果;实时RT-PCR;风险监测;

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