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首页> 外文期刊>Food microbiology >Effects of pulsed light and sanitizer wash combination on inactivation of Escherichia coli O157:H7, microbial loads and apparent quality of spinach leaves
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Effects of pulsed light and sanitizer wash combination on inactivation of Escherichia coli O157:H7, microbial loads and apparent quality of spinach leaves

机译：脉冲光和洗脱液剂洗涤组合对大肠杆菌灭活的影响：H7，微生物载荷和菠菜叶片的表观品质

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摘要

The purpose of this study was to investigate the efficacy of pulsed light (PL), a new formula of sanitizer (HEN) consisting of hydrogen peroxide, EDTA and Nisin, as well as synergy of PL and HEN sanitizer (PL-HEN) wash in inactivating E. coli O157:H7 on spinach. The treatment effect on microbial loads and apparent quality during 13 days storage at 4 degrees C was also determined. A bacterial cocktail containing three strains of E. coli O157:H7 was used as inoculum based on their association with produce-related outbreaks. Spinach leaves were spot inoculated on surface before treating with PL (1-63J/cm(2)), HEN sanitizer wash (2 min) or their combinations. PL in-activation was influenced significantly at low doses. Treatment dose of 15.75 J/cm(2), equivalent to 15 s intense PL treatment, was found optimal above which adverse quality effect was evident. The optimal PL dose resulted 2.7 log CFU/g reduction of E. coli O157:H7 while a rapid 2 min wash in sanitizer formulation HEN, provided comparatively low, 1.8 log CFU/g, reduction of the pathogen. Two different sequences of PL and HEN treatment combinations were tested. In PL-HEN treatment, inoculated leaves were first treated at optimal PL dose (15.75 J/cm(2)) followed by 2 min immersion in HEN whereas in HEN-PL treatment, leaves were first washed in HEN before PL exposure. HEN-PL treatment indicated a compound inactivation activity (4.6 logs reduction) while PL-HEN treatment indicated a strong synergistic inactivation as E. coli cells were not detectable after treatment indicating 5 log reduction. The PL-HEN treatment not only significantly reduced spoilage microbial populations on spinach but also slowed their growth during storage. Furthermore, the visual and firmness quality of spinach were not significantly affected by the PL-HEN treatment. Overall, our results demonstrate that integrated PL-HEN technology can be used to enhance microbial safety of spinach.
机译：本研究的目的是研究脉冲光（PL），消毒剂（母鸡）的新配方，由过氧化氢，EDTA和NISIN组成的疗效，以及PL和HEN SUNITIZER（PL-HEN）洗涤的协同作用在菠菜上灭活大肠杆菌O157：H7。还确定了对4℃的13天储存期间微生物载荷和表观质量的治疗效果。一种含有三种大肠杆菌O157：H7菌株的细菌鸡尾酒基于与产生相关爆发的关联。用PL（1-63J / cm（2）），母鸡消毒剂洗涤（2分钟）或其组合，在表面接种在表面上接种在表面上。在低剂量下，Pl活化是显着的影响。治疗剂量为15.75J / cm（2），相当于15秒的肌肉处理，得到最佳的优质效果显而易见。最佳PL剂量引发2.7测定大肠杆菌O157：H7的测定CFU / g的降低，而在洗脱液剂制剂母鸡中快速2分钟洗涤，提供相对较低，1.8对数CFU / g，降低病原体。测试了两种不同的PL和母鸡处理组合序列。在PL-HEN处理中，在最佳PL剂量（15.75J / cm（2））下首先处理接种叶子，然后在母鸡处理中浸入2分钟，而在PL暴露前首先用叶片洗涤叶。 HEN-PL处理表明复合灭活活性（4.6降低），而PL-HEN处理表明在治疗后未检测到大肠杆菌细胞的强烈协同灭活，表明> 5降低物降低。 PL-Hen治疗不仅显着降低了菠菜的腐败微生物群，而且在储存期间也减缓了它们的生长。此外，菠菜的视觉和坚固质量受到PL-HEN处理的显着影响。总体而言，我们的结果表明，集成的PL-Hen技术可用于增强菠菜的微生物安全性。

著录项

  • 来源
    《Food microbiology》 |2019年第9期|127-134|共8页
  • 作者

    Mukhopadhyay Sudarsan; Sokorai Kimberly; Ukuku Dike O.; Fan Xuetong; Olanya Modesto; Juneja Vijay;

  • 作者单位

    ARS Residue Chem & Predict Microbiol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

    ARS Residue Chem & Predict Microbiol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

    ARS Food Safety Intervent Technol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

    ARS Residue Chem & Predict Microbiol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

    ARS Food Safety Intervent Technol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

    ARS Residue Chem & Predict Microbiol Res Unit USDA Eastern Reg Res Ctr Wyndmoor PA 19038 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Escherichia coli O157:H7; Spinach; Pulsed light; Sanitizer; Microbial load; Quality;

    机译：大肠杆菌O157：H7;菠菜;脉冲光;消毒剂;微生物载荷;质量;

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