• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Detection of enterohaemorrhagic Escherichia coli in food by droplet digital PCR to detect simultaneous virulence factors in a single genome
【24h】

Detection of enterohaemorrhagic Escherichia coli in food by droplet digital PCR to detect simultaneous virulence factors in a single genome

机译：通过液滴数量PCR检测食物中的肠状鼠肠大肠杆菌检测单个基因组同时毒力因子

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Shiga toxin producing E. coli ate a problem for food producers. STEC's require a combination of virulence factors to cause disease, so ideally detection techniques should detect the presence of multiple virulence factors in a single cell directly from food. Droplet Digital PCR (ddPCR) is commonly used to quantify the number of copies of a gene in a sample, moreover it is able to link two genes to the same piece of DNA. Here stx and an O-antigen specific gene are detected simultaneously with taqman probes confirming that the cells are intact as well as distinguishing between strains based on their genotype. Using ddPCR E. coli O157.H7 and O104:H4 are quantified from apple juice, milk and spinach washings without an enrichment step, the detection limit of ddPCR in apple juice was 2 cfu/mL. Also, ddPCR was used to detect pathogenic bacterial cells in the presence of background strains which shared one or none of the target genes, including avirulent strains. Whole cell ddPCR is compared to several DNA extraction techniques demonstrating that whole cell ddPCR is more reliable for linking genes within an organism. Whole cell ddPCR is a promising technique for the rapid and specific detection of foodborne pathogens.
机译：Shiga毒素生产大肠杆菌对食品生产者的问题。 STEC需要组合毒力因子来引起疾病，因此理想的检测技术应直接从食物中检测单个细胞中多种毒力因子的存在。液滴数码PCR（DDPCR）通常用于量化样品中基因的拷贝数，此外，它能够将两个基因链接到同一块DNA。这里与Taqman探针同时检测STX和O-抗原特异性基因，证实细胞是完整的，并且基于其基因型区分菌株。使用DDPCR大肠杆菌O157.H7和O104：H4通过苹果汁，牛奶和菠菜洗涤量化而没有富集步骤，苹果汁中DDPCR的检测极限为2 CFU / mL。此外，DDPCR用于检测致病细菌细胞，在存在一个或非靶基因的背景菌株存在下，包括无毒菌株。将整个细胞DDPCR与若干DNA提取技术进行比较，证明整个细胞DDPCR更可靠用于将基因连接在生物体内。全细胞DDPCR是一种有希望的技术，用于快速和特异性检测食源性病原体。

著录项

  • 来源
    《Food microbiology》 |2020年第9期|103466.1-103466.10|共10页
  • 作者

    Li He; David J Simpson; Michael G. Gaenzle;

  • 作者单位

    University of Alberta Department of Agricultural Food and Nutritional Science Canada Sichuan Agricultural University College of Food Science Ya'an Sichuan PR China;

    University of Alberta Department of Agricultural Food and Nutritional Science Canada;

    University of Alberta Department of Agricultural Food and Nutritional Science Canada Hubei University of Technology College of Bioengineering and Food Science Wuhan Hubei PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Droplet digital PCR; EHEC; Pathogen detection; Shiga toxin;

    机译：液滴数码PCR;EHEC;病原体检测;滋阴毒素;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号