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首页> 外文期刊>Food microbiology >A loop-mediated isothermal amplification (LAMP) assay for rapid detection of fumonisin producing Aspergillus species
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A loop-mediated isothermal amplification (LAMP) assay for rapid detection of fumonisin producing Aspergillus species

机译：一种环介导的等温扩增（灯）测定，用于快速检测生产曲霉菌毒素物种

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摘要

Fumonisins contamination of food commodities is a worldwide problem, especially for maize. The ability to produce fumonisinsis a trait of several species of Fusarium, mainly F. verticillioides and F. proliferatum on maize, and some Aspergillus species. A. niger and its sister species A. welwitschiae, can contribute to fumonisin B_2 (FB_2) accumulation in maize kernels, although to a lesser extent than fumonisin-producing Fusarium species. Fumonisins risk monitoring represents an effective strategy in the integrated approach for mycotoxin risk management and reduction. The availability of a user-friendlymolecular assay for the detection oftoxigenic fungal species represents a valuable tool in understanding and managing upcoming mycotoxin contamination. In this study, we developed a LAMP assay, based on the detection of fum10, for a rapid and specific molecular detection of FB_2-producing A. niger and A. welwistchiae, potentially useful to perform monitoring directly "on site" in maize chain. Results showed that very low amounts of conidia are suitable to detect the presence of the target gene, thus providing information about the presence of FB_2-producing Aspergtillus species and the possible upcoming fumonisins contamination in maize. The assay was combined with a suitable protocol for "in field" crude DNA extraction and a colorimetric method for easy naked-eye evaluationof results, offering a reliable and user-friendly tool to support effective reduction strategies of mycotoxin contamination in crop management programs.
机译：Fumonisins污染食品商品是全球问题，特别是对于玉米而言。生产Fumonisinsis的能力是几种镰刀菌的特征，主要是F. Verticillioides和玉米的F.Veriferatum，以及一些曲霉属。 A.尼日尔及其姐妹种类A. Welwitschiae可以促进玉米内核中的Fumonisin B_2（FB_2）积累，尽管在较小程度上，比产生富马敏的镰刀菌种。 Fumonisins风险监测是霉菌毒素风险管理和减少综合方法的有效策略。用于检测的人友好分子测定的可用性用于氧化真菌物种的理解和管理即将到来的霉菌毒素污染的有价值的工具。在这项研究中，我们开发了一种基于FUM10的检测的灯测定，用于快速和特异性的FB_2生产A.尼日尔和A.WERWISTCHIAE的分子检测，可能有助于在玉米链中直接进行监测。结果表明，非常少量的分类是适合于检测靶基因的存在，从而提供有关产生FB_2的存在的信息和玉米中可能的即将到来的富马菌素污染。该测定与“野外”粗DNA提取的合适方案组合，并为易肉眼评估的比例方法，提供了可靠和用户友好的工具，以支持在作物管理计划中的霉菌毒素污染的有效减少策略。

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  • 来源
    《Food microbiology》 |2020年第9期|103469.1-103469.7|共7页
  • 作者

    Massimo Ferrara; Antonio F. Logrieco; Antonio Moretti; Antonia Susca;

  • 作者单位

    Institute of Sciences of Food Production (ISPA) National Research Council (CNR) Ban Italy;

    Institute of Sciences of Food Production (ISPA) National Research Council (CNR) Ban Italy;

    Institute of Sciences of Food Production (ISPA) National Research Council (CNR) Ban Italy;

    Institute of Sciences of Food Production (ISPA) National Research Council (CNR) Ban Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aspergillus niger; Aspergillus welwitschiae; Rapid molecular tool; Fumonisin; Maize;

    机译：曲霉;aspergillus welwitschiae;快速分子工具;Fumonisin;玉米;

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