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Novel real-time PCR assay for Lactobacillus casei group species using comparative genomics

机译：使用比较基因组学的乳酸杆菌组种类的新型实时PCR测定

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摘要

The Lactobacillus casei group, which includes the closely related species L. casei, L. paracasei, L. rhamnosus, and L. chiayiensis, has been under debate regarding its taxonomy because of the difficulty in distinguishing the species from each other. In the present study, we developed a novel real-time PCR assay for distinguishing the L. casei group species. The pan-genome, as determined by the genomes of 44 strains, comprised 6789 genes, comparative genomic analysis showed that L. casei group strains were classified by species. Based on these results, species-specific genes were identified, and primers were designed from those genes. Real-time PCR clearly distinguished each species of the L. casei group and specifically amplified only to the target species. The method was applied to 29 probiotic products, and the detected results and label claims were compared. Total 23 products were in accordance with the label claims, and the remaining products contained species different from those stated in the label claims. Our method can rapidly and accurately distinguish the L. casei group species in a single reaction. Hence, our assay can be applied to identify L. casei group species from food or environmental samples and to accurately determine the nomenclature of the species.
机译：乳酸杆菌组，其中包括密切相关的物种L.酪虫，L. paracasei，L. rhamnosus和L.Chiayiensis，这一直是关于其分类物的辩论，因为难以区分物种。在本研究中，我们开发了一种用于区分L.酪虫组种类的新型实时PCR测定。由44个菌株的基因组确定的泛基因组包含6789个基因，比较基因组分析显示L.酪菌群菌株被物种分类。基于这些结果，鉴定了特异性基因，并从这些基因设计了引物。实时PCR清楚地区分L.酪虫组的每种物种，仅对靶物种特异性扩增。将该方法应用于29种益生菌产物，并比较了检测结果和标记要求。总共23种产品均符合标签要求，其余产品包含与标签索赔中所述的那些不同的物种。我们的方法可以在单一反应中迅速和准确地区分L.酪菌群物种。因此，我们的测定可以应用于从食物或环境样本中鉴定L.酪菌组种类，并准确地确定物种的命名。

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  • 来源
    《Food microbiology》 |2020年第9期|103485.1-103485.9|共9页
  • 作者

    Eiseul Kim; Seung-Min Yang; Eun-Ji Cho; Hae-Yeong Kim;

  • 作者单位

    Institute of Life Sciences and Resources and Department of Food Science and Biotechnology Kyung Hee University Yongin 17104 South Korea;

    Institute of Life Sciences and Resources and Department of Food Science and Biotechnology Kyung Hee University Yongin 17104 South Korea;

    Institute of Life Sciences and Resources and Department of Food Science and Biotechnology Kyung Hee University Yongin 17104 South Korea;

    Institute of Life Sciences and Resources and Department of Food Science and Biotechnology Kyung Hee University Yongin 17104 South Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lactobacillus casei group; Comparative genomics; Probiotic products; Real-time PCR; Species-specific primer;

    机译：乳酸杆菌酪虫组;比较基因组学;益生菌产品;实时PCR;物种特异性底漆;

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