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Virological analyses in collective catering outbreaks in France between 2012 and 2017

机译:2012年和2017年在法国集体餐饮疫情中的病毒学分析

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摘要

Enteric viruses cause the majority of foodborne illnesses and common symptoms of many foodborne illnesses include vomiting, diarrhea, abdominal pain, and fever. Among the enteric viruses, human Norovirus (NoV) and hepatitis virus (HAV and HEV) are the main viruses suspected to cause foodborne outbreaks and represent a serious public health. The study presents survey tools of viruses in a wide variety of foodstuffs and results obtained during 56 foodborne outbreaks investigation in France between 2012 and 2017. 246 suspected foods were examined for the presence of four human enteric viruses (NoV GⅠ and NoV GⅡ, HAV or HEV) either using methods described in the EN ISO 15216-1 or in house methods. All viral analysis of food samples were performed with the implementation of process control and an external amplification controls. Eighteen of 56 foodborne outbreaks investigated included at least one positive food sample (16/18 NoV, 1/18 HAV and 1/18 HEV). The genomic levels of four viruses detected ranged from < 10~2 to 10~7 genome copies per g or per L This study showed the interest to develop methods for the extraction of viruses in different foodstuffs to increase the possibility to identify the association between viral illness and food consumption.
机译:肠道病毒导致大多数食物中的疾病和许多食物中疾病的常见症状包括呕吐,腹泻,腹痛和发烧。在肠溶病毒中,人类诺罗病毒(11月)和肝炎病毒(HAV和HEV)是怀疑引起食源性爆发的主要病毒并代表着严重的公共卫生。该研究提出了在2012年和2017年间法国的各种食品中的病毒病毒的调查工具,并在2012年和2017年之间进行了56名食源性爆发调查。检查了446例疑似食物,用于四种人类肠道病毒(11月GⅠ和11月GⅡ,HAV或HEV)使用EN ISO 15216-1或HUSE方法中描述的方法。通过实施过程控制和外部扩增对照进行食物样品的所有病毒分析。 56个食物中爆发的十八次调查包括至少一个正面食品样本(11月16日,1月18日,1/18 Hav和1/18 HEV)。检测到的四种病毒的基因组水平范围为<10〜2至10〜7个基因组拷贝,本研究表明,开发用于提取不同食品中病毒的方法的兴趣,以增加鉴定病毒之间的关联的可能性疾病和食品消费。

著录项

  • 来源
    《Food microbiology》 |2020年第10期|103546.1-103546.9|共9页
  • 作者单位

    University Paris-Est ANSES Laboratory for food Safety F-94700 Maisons-Alfort France;

    University Paris-Est ANSES Laboratory for food Safety F-94700 Maisons-Alfort France;

    University Paris-Est ANSES Laboratory for food Safety F-94700 Maisons-Alfort France;

    University Paris-Est ANSES Laboratory for food Safety F-94700 Maisons-Alfort France;

    University Paris-Est ANSES Laboratory for food Safety F-94700 Maisons-Alfort France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Foodborne outbreaks; Human norovirus; Hepatitis virus (AE) RT-qPCR;

    机译:食源性爆发;人类诺维病毒;肝炎病毒(AE)RT-QPCR;

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