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首页> 外文期刊>Food microbiology >Inhibition of Listeria monocytogenes by the Staphylococcus capitis - derived bacteriocin capidermicin
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Inhibition of Listeria monocytogenes by the Staphylococcus capitis - derived bacteriocin capidermicin

机译：用葡萄球菌的葡萄球菌菌毛炎李斯特菌单核细胞生成的抑制细菌霉素

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摘要

Natural methods to control food pathogens are required and bacteriocins have received much interest in this regard. The aim of this study was to investigate the ability of the novel bacteriocin capidermicin to inhibit Listeria monocytogenes. Agar-based deferred antagonism assays were carried out with the capidermicin producer against 17 L. monocytogenes strains and large zones of inhibition were observed for 12 strains. Minimal inhibitory concentration assays performed with purified capidermicin peptide revealed MIC values between 680 nM and 11 μM. Biofilm assays were performed with five L. monocytogenes strains. Addition of capidermicin prevented biofilm formation by one strain and could remove pre-established biofilms of all five strains. Broth based growth experiments demonstrated that addition of capidermicin resulted in an extended lag phase of both L. monocytogenes strains tested. Kill-curve experiments showed that capidermicin was able to potentiate the anti-Listeria effects of the lantibiotic nisin. This enhanced killing by the combination of both peptides was also observed in model food systems (cottage cheese and chocolate milk). In summary, we show that capidermicin can inhibit L. monocytogenes and warrants further investigation as a potential natural agent for the control of this pathogen.
机译：控制食品病原体的天然方法是必需的，并且细菌病在这方面得到了很多兴趣。本研究的目的是探讨新型细菌素Capidermicin抑制histeria单核细胞增生的能力。将基于琼脂的渗透拮抗拮抗拮抗测定法用Capidermicin生产者进行，针对17L.单核细胞生成的菌株和大区被观察到12个菌株。用纯化的Capidermin肽进行的最小抑制浓度测定显示出680nm和11μm之间的MIC值。生物膜测定用五升单核细胞元菌株进行。加入藜霉素通过一种菌株预防生物膜形成，可以除去所有五种菌株的预先成立的生物膜。基于肉汤的生长实验表明，加入藜霉素导致延长的L.单核细胞增生菌株的延长滞后阶段。杀灭曲线实验表明，Capidermicin能够提高毫菌硝基苯的抗李斯特菌。在模型食品系统（奶酪和巧克力牛奶）中也观察到这种通过两种肽组合的增强杀伤。总之，我们表明Capidermicin可以抑制L.单核细胞增生，并保证进一步调查作为控制该病原体的潜在天然剂。

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  • 来源
    《Food microbiology》 |2021年第4期|103661.1-103661.11|共11页
  • 作者

    David Lynch; Colin Hill; Des Field; Maire Begley;

  • 作者单位

    Department of Biological Sciences Cork Institute of Technology Cork Ireland;

    School of Microbiology University College Cork Cork Ireland APC Microbiome Ireland University College Cork Ireland;

    School of Microbiology University College Cork Cork Ireland APC Microbiome Ireland University College Cork Ireland;

    Department of Biological Sciences Cork Institute of Technology Cork Ireland APC Microbiome Ireland University College Cork Ireland;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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