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首页> 外文期刊>Food microbiology >Restoration of GABA production machinery in Lactobacillus brevis by accessible carbohydrates, anaerobiosis and early acidification
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Restoration of GABA production machinery in Lactobacillus brevis by accessible carbohydrates, anaerobiosis and early acidification

机译：通过可利用的碳水化合物，厌氧菌和早期酸化来恢复短乳杆菌中的GABA生产机制

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摘要

Lactobacillus brevis is an efficient cell factory for producing bioactive γ-aminobutyric acid (GABA) by its gad operon-encoded glutamic acid decarboxylase (GAD) system. However, little mechanistic insights have been reported on the effects of carbohydrate, oxygen and early acidification on GABA production machinery in Lb. brevis. In the present study, GABA production from Lb. brevis was enhanced by accessible carbohydrates. Fast growth of this organism was stimulated by maltose and xylose. However, its GABA production was highly suppressed by oxygen exposure, but was fully restored by anaerobiosis that up-regulated the expression of gad operon in Lb. brevis cells. Although the level of cytosolic acidity was suitable for the functioning of GadA and GadB, early acidification of the medium (ipH 5 and ipH 4) restored GABA synthesis strictly in aerated cells of Lb. brevis because the expression of gad operon was not up-regulated in them. We conclude that GABA production machinery in Lb. brevis could be restored by accessible carbohydrates, anaerobiosis and early acidification. This will be of interest for controlling fermentation for synthesis of GABA and manufacturing GABA-rich fermented vegetables.
机译：短乳杆菌是通过其操纵子编码的谷氨酸脱羧酶（GAD）系统生产生物活性γ-氨基丁酸（GABA）的高效细胞工厂。然而，关于碳水化合物，氧气和早期酸化对Lb中GABA生产机制的影响的机械机理见解很少。布雷维斯。在本研究中，Lb生产GABA。可食用的碳水化合物增强了灯盏花。麦芽糖和木糖刺激该生物的快速生长。然而，其GABA的产生被氧气暴露高度抑制，但被厌氧菌完全恢复，而厌氧菌上调了Lb中gad操纵子的表达。灯盏花细胞。尽管胞浆酸度水平适合于GadA和GadB的功能，但培养基的早期酸化（ipH 5和ipH 4）严格恢复了Lb充气细胞中GABA的合成。 brevis是因为它们中的gad操纵子的表达没有上调。我们得出结论，LABA中的GABA生产机械。短纤维可以通过可利用的碳水化合物，厌氧菌和早期酸化来恢复。这对于控制用于合成GABA的发酵和生产富含GABA的发酵蔬菜将是有意义的。

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  • 来源
    《Food microbiology》 |2018年第2期|151-158|共8页
  • 作者

    Wu Qinglong; Shah Nagendra P.;

  • 作者单位

    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong,Department of Pathology and Immunology & Texas Children's Microbiome Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, United States;

    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Acid resistance; Carbohydrate; GABA; Lactobacillus brevis; Oxygen;

    机译：耐酸性糖类;GABA;短乳杆菌;氧;

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