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首页> 外文期刊>Food microbiology >A novel approach in acidic disinfection through inhibition of acid resistance mechanisms; Maleic acid-mediated inhibition of glutamate decarboxylase activity enhances acid sensitivity of Listeria monocytogenes
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A novel approach in acidic disinfection through inhibition of acid resistance mechanisms; Maleic acid-mediated inhibition of glutamate decarboxylase activity enhances acid sensitivity of Listeria monocytogenes

机译：通过抑制抗酸机制在酸性消毒中的新方法；马来酸介导的谷氨酸脱羧酶活性抑制增强单核细胞增多性李斯特菌的酸敏感性

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摘要

Here it is demonstrated a novel approach in disinfection regimes where specific molecular acid resistance systems are inhibited aiming to eliminate microorganisms under acidic conditions. Despite the importance of the Glutamate Decarboxylase (GAD) system for survival of Listeria monocytogenes and other pathogens under acidic conditions, its potential inhibition by specific compounds that could lead to its elimination from foods or food preparation premises has not been studied. The effects of maleic acid on the acid resistance of L. monocytogenes were investigated and found that it has a higher antimicrobial activity under acidic conditions than other organic acids, while this could not be explained by its pKa or Ka values. The effects were found to be more pronounced on strains with higher GAD activity. Maleic acid affected the extracellular GABA levels while it did not affect the intracellular ones. Maleic acid had a major impact mainly on GadD2 activity as also shown in cell lysates. Furthermore, it was demonstrated that maleic acid is able to partly remove biofilms of L. monocytogenes. Maleic acid is able to inhibit the GAD of L. monocytogenes significantly enhancing its sensitivity to acidic conditions and together with its ability to remove biofilms, make a good candidate for disinfection regimes.
机译：在此证明了一种消毒方法的新颖方法，其中特定的分子酸抗性系统被抑制，旨在消除酸性条件下的微生物。尽管谷氨酸脱羧酶（GAD）系统对于酸性条件下单核细胞增生李斯特氏菌和其他病原体的存活很重要，但尚未研究其对特定化合物的潜在抑制作用，这种抑制作用可能导致其从食品或食品制备场所中清除。研究了马来酸对单核细胞增生李斯特氏菌的耐酸性的影响，发现它在酸性条件下具有比其他有机酸更高的抗菌活性，而这不能用其pKa或Ka值来解释。发现对具有较高GAD活性的菌株的作用更为明显。马来酸影响细胞外GABA水平，但不影响细胞内GABA水平。马来酸主要对GadD2活性有重要影响，如细胞裂解液中所示。此外，已证明马来酸能够部分去除单核细胞增生李斯特菌的生物膜。马来酸能够抑制单核细胞增生李斯特菌的GAD，从而显着增强其对酸性条件的敏感性，并具有去除生物膜的能力，是消毒方案的良好候选者。

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  • 来源
    《Food microbiology》 |2018年第2期|96-104|共9页
  • 作者

    Paudyal Ranju; Barnes Ruth H.; Karatzas Kimon Andreas G.;

  • 作者单位

    Department of Food and Nutritional Sciences, The University of Reading, PO Box 226, Whiteknights, Reading, United Kingdom;

    Department of Food and Nutritional Sciences, The University of Reading, PO Box 226, Whiteknights, Reading, United Kingdom;

    Department of Food and Nutritional Sciences, The University of Reading, PO Box 226, Whiteknights, Reading, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biofilm; GAD; Glutamate decarboxylase; Inhibition; Listeria monocytogenes; Maleic acid;

    机译：生物膜GAD;谷氨酸脱羧酶;抑制;李斯特菌;马来酸;

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