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首页> 外文期刊>Food microbiology >Temperature effects on the growth and survival of tdh positive Vibrio parahaemolyticus in tissues of postharvest Manila clam (Ruditapes philippinarum)
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Temperature effects on the growth and survival of tdh positive Vibrio parahaemolyticus in tissues of postharvest Manila clam (Ruditapes philippinarum)

机译：温度对马尼拉蛤（Ruditapes philippinarum）收获后tdh阳性副溶血性弧菌生长和存活的影响

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摘要

Bivalve storage at inadequate temperatures contains greater densities ofVibrio parahaemolyticus. The objective of the present study was to determine if there were any differences in growth and survival of potentially pathogenicV. parahaemolyticus(tdhpositive) in relation to nonpathogenicV. parahaemolyticus(tdh-trhnegative) levels in Manila clams (Ruditapes philippinarum) when exposed to different postharvest temperatures. Clams were depurated then exposed to known doses of both potential pathogenic and nonpathogenicV. parahaemolyticusfor 24 h. Clams were then kept at the following temperatures corresponding to the Mediterranean summer (28 °C), winter (15 °C) and refrigeration (4 °C) for 96 h.Vibrio parahaemolyticusdensities were determined at 0, 24, 48, 72 and 96 h of postharvest storage. Both isolates ofV. parahaemolyticusmultiplied rapidly in live clams held at 28 °C, (increase of 3 logs CFU/g at 72 h). NonpathogenicV. parahaemolyticusin clams stored at 4 °C and 15 °C showed 1 log CFU/g and 2 log CFU/g decrease at 96 h, respectively, while no significant differences were detected for pathogenicV. parahaemolyticusat these temperatures after 96 h.
机译：在不适当的温度下双壳类动物的储存含有更高密度的副溶血性弧菌。本研究的目的是确定潜在致病性V的生长和存活是否存在任何差异。与非致病性有关的parahaemolyticus（tdhpositive）V.当暴露于不同的收获后温度时，马尼拉蛤（Ruditapes philippinarum）中的副溶血（tdh-trhnegative）水平。蛤被净化，然后暴露于已知剂量的潜在致病性和非致病性V。副溶血24小时然后将蛤at保持在与地中海夏季（28°C），冬季（15°C）和冷藏（4°C）相对应的以下温度下96小时。确定副溶血性弧菌的密度为0、24、48、72和96收获后的储存时间。 V的两个分离株。副溶血菌在保持28°C的活蛤中迅速繁殖（72小时时增加3 log CFU / g）。非致病性分别在4°C和15°C储存的溶血副溶血性蛤在96 h时分别下降1 log CFU / g和2 log CFU / g，而致病性V却没有显着差异。在96h后在这些温度下进行副溶血。

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