Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food

Martin Spanoghe; Mario Godoy Jara; John Riviere; Deborah Lanterbecq; Martine Gadenne; Thierry Marique

首页> 外文期刊>Food microbiology >Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food

【24h】

Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food

机译：快速定量检测食品中多面酵母Kazachstania servazzii的定量实时PCR分析方法的开发和应用

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The beneficial contributions af Kazachstania servazzii are well-established in various food processes. This yeast also contributes in the spoilage of finished packaged food due to abundant gas production. In particular, an occurrence of K servazzii was recently positively correlated with the formation of severe package swelling of some prepared fresh pizzas. To circumscribe this concern, a quantitative SYBR green real-time PCR assay based on a newly designed specific primer pair targeting the ribosomal ITS1-5.8S-ITS2 region of K. servazzii was developed. The quantification was enabled using a standard curve created from serially diluted plasmids containing the target sequence of the K. servazzii strain. A validation of the assay was achieved by enumeration of K. servazzii DNA copies from artificially infected culture broths containing non-contaminated pizza substrates. The newly developed method was then tested on total DNA extracted from packaged fresh pizzas, in which certain lots were swollen and thus suspected of containing K. servazzii. This study highlights that this newly developed quantitative assay is not only sufficiently sensitive, specific and reliable to be functionally used in food control as a routine method of detection, but also promising in specific studies that seek to further characterize the dynamic of this yeast in some increasingly popular food processes.

机译：Kazachstania servazzii的有益贡献在各种食品加工中都得到了公认。由于大量的气体产生，该酵母还导致成品包装食品变质。特别是，最近出现的K servazzii与一些准备好的新鲜比萨的严重包装膨胀形成正相关。为了解决这一问题，开发了一种基于新设计的，针对SERVKii核糖体ITS1-5.8S-ITS2区域的特异性引物对的SYBR绿色实时荧光定量PCR分析方法。使用标准曲线来进行定量，该标准曲线是由包含SERVSERVii菌株靶序列的连续稀释质粒创建的。通过枚举来自人工感染的含有未污染比萨饼底物的培养液中的SERVK ii DNA拷贝来完成测定的验证。然后，对从包装的新鲜比萨中提取的总DNA进行了新开发的方法的测试，其中某些批料已肿胀，因此怀疑含有K. servazzii。这项研究强调，这种新开发的定量测定方法不仅足够灵敏，特异性和可靠，可以在食品控制中用作常规检测方法，而且在旨在进一步表征某些酵母菌动态特性的特定研究中很有希望越来越受欢迎的食品加工。

著录项

来源
《Food microbiology》 |2017年第4期|133-140|共8页
作者
Martin Spanoghe; Mario Godoy Jara; John Riviere; Deborah Lanterbecq; Martine Gadenne; Thierry Marique;
展开▼
作者单位

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciale de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium,Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 11, rue Paul Pastur, 7800 Ath, Wallonia, Belgium;

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium,Centre pour l'Agronomie et l'Agro-Industrie de la Province de Hainaut (CARAH), 11 rue Paul Pastur, 7800, Ath, Wallonia, Belgium;

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium;

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium;

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium,Centre pour l'Agronomie et l'Agro-Industrie de la Province de Hainaut (CARAH), 11 rue Paul Pastur, 7800, Ath, Wallonia, Belgium;

Laboratory of Biotechnology and Applied Biology, Haute Ecole Provinciate de Hainaut-CONDORCET, 17 Chemin du Champ de Mars, 7000 Mons, Wallonia, Belgium;

展开▼
收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Saccharomycetaceae; qPCR detection; Package swelling; Spoilage; Food; Pizza;

机译：酿酒科;qPCR检测;包装膨胀;损坏;餐饮;比萨;

相似文献

外文文献
中文文献
专利

1. Development of a rapid and reliable TaqMan probe-based real-time PCR assay for the detection and enumeration of the multifaceted yeast Kluyveromyces marxianus in dairy products [J] . Kim Dong-Hyeon, Jeong Dana, Kang Il-Byeong, LWT-Food Science & Technology . 2018,第期

机译：开发快速可靠的Taqman探针的实时PCR测定，用于检测和枚举乳制品中Marxianus的多方面酵母Kluyveromyces Marxianus
2. Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products [J] . Gianluca Bleve, Lucia Rizzotti, Franco Dellaglio, Applied and Environmental Microbiology . 2003,第7期

机译：逆转录（RT）-PCR和实时RT-PCR分析方法的开发，用于快速检测和定量污染酸奶和巴氏杀菌食品的活酵母和霉菌
3. Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products [J] . Gianluca Bleve, Lucia Rizzotti, Franco Dellaglio, Applied and Environmental Microbiology . 2003,第7期

机译：逆转录（RT）-PCR和实时RT-PCR分析方法的开发，用于快速检测和定量污染酸奶和巴氏杀菌食品的活酵母和霉菌
4. Development of a Foodborne Bacterial Pathogen PCR Electrospray Ionization Mass Spectrometry Biosensor Detection Assay for Rapid, High-Throughput Food Testing [C] . Erik Burrows, Ranga Sampath, S. Allard, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：开发食品枯竭的细菌病原体PCR电喷雾电离质谱生物传感器检测测定，用于快速，高通量食物检测
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products [O] . Gianluca Bleve, Lucia Rizzotti, Franco Dellaglio, 2003

机译：逆转录（RT）-PCR和实时RT-PCR分析方法的开发用于快速检测和定量污染酸奶和巴氏杀菌食品的活酵母和霉菌
7. Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products [O] . Bleve, Gianluca, Rizzotti, Lucia, Dellaglio, Franco, 2003

机译：逆转录（RT）-PCR和实时RT-PCR分析方法的开发，用于快速检测和定量污染酸奶和巴氏杀菌食品的活酵母和霉菌

Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food

摘要

著录项

相似文献

相关主题

期刊订阅