• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation
【24h】

Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation

机译：开发一种结合MPN和qPCR并缩短培养时间的鼠伤寒沙门氏菌定量方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel method was developed for the specific quantification of S. Typhimurium using a most-probable-number (MPN) combined with qPCR and a shortened incubation time (MPN-qPCR-SIT). For S. Typhimurium enumeration, dilutions of samples were transferred into three wells on a microtiter plate and the plate was incubated for 4 h. The S. Typhimurium presence in the wells was identified using a qPCR and populations were determined based on an MPN calculation. The R2 between the MPN-qPCR-SIT and conventional MPN exhibited a high level of correlation (0.9335-0.9752), suggesting that the MPN-qPCR-SIT offers a reliable alternative method for S. Typhimurium quantification. Although plating and qPCR were limited in their ability to detect low levels of S. Typhimurium (e.g. 0.18 log MPN/ml), these levels could be successfully detected with the MPN-qPCR-SIT. Chicken breast samples inoculated with S. Typhimurium were incubated at 0, 4, and 24 h and incubated samples were subjected to microbiome analysis. Levels of Salmonella and Enterobacteriaceae increased significantly with incubation time. The obvious benefits of the MPN-qPCR-SIT are: 1) a further confirmation step is not required, 2) the detection limit is as low as conventional MPN, but 3) is more rapid, requiring approximately 7 h to simultaneously complete quantification.
机译：开发了一种新的方法，通过结合最大概率数（MPN）和qPCR和缩短的孵育时间（MPN-qPCR-SIT）来特异性鉴定鼠伤寒沙门氏菌。为了进行鼠伤寒沙门氏菌计数，将样品稀释液转移到微量滴定板上的三个孔中，并将板温育4小时。使用qPCR鉴定孔中鼠伤寒沙门氏菌的存在，并根据MPN计算确定种群。 MPN-qPCR-SIT和常规MPN之间的R2表现出很高的相关性（0.9335-0.9752），这表明MPN-qPCR-SIT为鼠伤寒沙门氏菌定量提供了可靠的替代方法。尽管铺板和qPCR检测鼠伤寒沙门氏菌水平较低（例如0.18 log MPN / ml）的能力受到限制，但可以使用MPN-qPCR-SIT成功检测到这些水平。接种鼠伤寒沙门氏菌的鸡胸肉样品分别在0、4和24 h孵育，并对孵育的样品进行微生物组分析。沙门氏菌和肠杆菌科的水平随着孵育时间的增加而显着增加。 MPN-qPCR-SIT的明显好处是：1）不需要进一步的确认步骤，2）检测限与常规MPN一样低，但是3）更快，需要大约7小时才能同时完成定量。

著录项

  • 来源
    《Food microbiology》 |2017年第8期|7-18|共12页
  • 作者

    Sun Ae Kim; Si Hong Park; Sang In Lee; Steven C. Ricke;

  • 作者单位

    Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA;

    Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA;

    Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA;

    Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Salmonella Typhimurium; Quantification; Detection; Rapid; Simple;

    机译：鼠伤寒沙门氏菌;量化;检测;快速;简单;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号