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首页> 外文期刊>Food microbiology >Molecular characterization and antimicrobial resistance of Salmonella enterica from swine slaughtered in two different types of Philippine abattoir
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Molecular characterization and antimicrobial resistance of Salmonella enterica from swine slaughtered in two different types of Philippine abattoir

机译：在两种不同类型的菲律宾屠宰场中宰杀的猪肠沙门氏菌的分子特征和抗药性

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摘要

Salmonella enterica is a well-known pathogen commonly acquired from the consumption of contaminated food. It has been estimated to affect millions of humans and cause hundreds of thousands of deaths per year globally. Pork, one of the most commonly consumed meats worldwide, has been identified as one of the main sources of human salmonellosis. In this study, we aimed to detect and characterize S. enterica from slaughtered swine and generate antimicrobial resistance profiles of select isolates. Tonsils and jejunum with mesenteric lymph nodes (MLN) were collected from a total of 240 swine from eight abattoirs (five accredited and three locally registered abattoirs) across Metro Manila. S. enterica were isolated using conventional culture methods and confirmed by PCR amplification of the invA gene. Isolates were further characterized based on somatic antigen by multiplex PCR. We report that there is no significant difference (P = 0.42) between the incidences of S. enterica in swine slaughtered in accredited (44.0%) and in locally registered abattoirs (46.7%). Most samples were contaminated with S. enterica under serogroup 0:3,10. Antimicrobial susceptibility testing of 183 isolates using the VITEK® 2 system revealed high resistance to ampicillin (67.8%) and trimethoprim/sulfamethoxazole (80.3%). Multidrug-resistance was found in 124 (67.8%) isolates.
机译：肠炎沙门氏菌是一种众所周知的病原体，通常是从食用受污染的食物中获得的。据估计，它每年影响全球数百万人，并造成数十万人死亡。猪肉是世界上最常用的肉类之一，已被确定为人类沙门氏菌病的主要来源之一。在这项研究中，我们旨在从屠宰的猪中检测和鉴定肠道链球菌，并产生精选菌株的抗药性。从马尼拉大都会的八只屠宰场（五只经鉴定的屠宰场和三只本地登记的屠宰场）的总共240头猪中收集了扁桃体和空肠及肠系膜淋巴结。用常规培养方法分离小肠链球菌，并通过PCR扩增invA基因确认。基于体抗原通过多重PCR进一步鉴定分离物。我们报告说，在认可的（44.0％）和当地注册的屠宰场（46.7％）屠宰的猪中，肠炎沙门氏菌的发病率之间没有显着差异（P = 0.42）。大多数样品在血清群0：3,10下均被肠炎链球菌污染。使用VITEK®2系统对183个分离株进行了抗菌药敏测试，结果显示对氨苄西林（67.8％）和甲氧苄啶/磺胺甲恶唑（80.3％）具有较高的耐药性。在124（67.8％）个分离物中发现了多药耐药性。

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  • 来源
    《Food microbiology》 |2017年第8期|51-56|共6页
  • 作者

    Alyzza Marie B. Calayag; bPhyllis Anne P. Paclibare; Pauline Dianne M. Santos; Corinne Aimee C. Bautista; Windell L. Rivera;

  • 作者单位

    Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

    Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

    Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

    Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

    Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

    Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City, 1101, Philippines;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Abattoir; Antimicrobial resistance; Salmonella enterica; Swine;

    机译：屠场抗菌素耐药性;肠沙门氏菌猪;

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