Reinforcement of Bacillus subtilis spores by cross-linking of outer coat proteins during maturation

Wishwas Abhyankar; Rachna Pandey; Alexander Ter Beek; Stanley Brul; Leo J. de Koning; Chris G. de Koster

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Reinforcement of Bacillus subtilis spores by cross-linking of outer coat proteins during maturation

机译：在成熟过程中通过外壳蛋白的交联增强枯草芽孢杆菌的孢子

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Resistance characteristics of bacterial endospores towards various environmental stresses such as chemicals and heat are in part attributed to their coat proteins. Heat resistance is developed in a late stage of sporulation and during maturation of released spores. Using our gel-free proteomic approach and LC-FT-ICR-MS/MS analysis we have monitored the efficiency of the tryptic digestion of proteins in the coat during spore maturation over a period of eight days, using metabolically ~(15)N labeled mature spores as reference. The results showed that during spore maturation the loss of digestion efficiency of outer coat and crust proteins synchronized with the increase in heat resistance. This implicates that spore maturation involves chemical cross-linking of outer coat and crust layer proteins leaving the inner coat layer proteins unmodified. It appears that digestion efficiencies of spore surface proteins can be linked to their location within the coat and crust layers. We also attempted to study a possible link between spore maturation and the observed heterogeneity in spore germination.

机译：细菌内生孢子对各种环境压力（例如化学物质和热量）的抗性特性部分归因于它们的外壳蛋白。在孢子形成的后期和释放的孢子成熟期间会产生耐热性。使用我们的无凝胶蛋白质组学方法和LC-FT-ICR-MS / MS分析，我们使用代谢〜（15）N标记了八天的时间，监测了孢子成熟过程中蛋壳中蛋白质的胰蛋白酶消化效率。成熟的孢子作为参考。结果表明，在孢子成熟过程中，外皮和地壳蛋白消化效率的损失与耐热性的增加同步。这意味着孢子成熟涉及外层和壳层蛋白的化学交联，而内层蛋白未修饰。看来，孢子表面蛋白的消化效率可以与其在外皮和硬皮层中的位置有关。我们还尝试研究孢子成熟与孢子萌发中观察到的异质性之间的可能联系。

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来源
《Food microbiology》 |2015年第febaptaa期|54-62|共9页
作者
Wishwas Abhyankar; Rachna Pandey; Alexander Ter Beek; Stanley Brul; Leo J. de Koning; Chris G. de Koster;
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作者单位

Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands,Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands,Room C2.263, Science Park 904, 1098 XH Amsterdam, The Netherlands;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Bacillus subtilis; Spore maturation; Spore coat; Protein cross-linking; LC-FT-ICR-MS/MS; Thermal resistance; Germination;

机译：枯草芽孢杆菌孢子成熟;孢子皮;蛋白质交联;LC-FT-ICR-MS / MS;热阻;发芽;

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专利

1. CotM of Bacillus subtilis, a member of the alpha-crystallin family of stress proteins, is induced during development and participates in spore outer coat formation. [J] . A O Henriques, B W Beall, C P Moran Journal of bacteriology . 1997,第6期

机译：枯草芽孢杆菌的CotM是应激蛋白的α-晶状体蛋白家族的成员，在发育过程中被诱导并参与孢子外皮的形成。
2. SpoVID functions as a non‐competitive hub that connects the modules for assembly of the inner and outer spore coat layers in Bacillus subtilis Bacillus subtilis [J] . Nunes Filipa, Fernandes Catarina, Freitas Carolina, Molecular Microbiology . 2018,第4期

机译：Spovid用作非竞争力的集线器，连接模块，用于组装内部和外孢子涂层枯草芽孢杆菌枯草芽孢杆菌
3. The spore coat is essential for Bacillus subtilis spore resistance to pulsed light, and pulsed light treatment eliminates some spore coat proteins [J] . International Journal of Food Microbiology . 2020,第期

机译：孢子涂层对于枯草芽孢杆菌孢子抗脉冲光，脉冲涂层是必不可少的，并且脉冲光处理消除了一些孢子涂层蛋白质
4. Functional display of target proteins on Bacillus subtilis spore and its application [C] . Byung-Gee Kim China-Japan-Korea joint symposium on enzyme engineering . 2010

机译：靶蛋白在枯草芽孢杆菌孢子上的功能展示及其应用
5. Identification of a new protein involved in Bacillus subtilis spore germination and study of its gene expression, localization and cross-linking into the spore coat. [D] . Ragkousi, Katerina. 2004

机译：鉴定一种新的参与枯草芽孢杆菌孢子萌发的蛋白质，并研究其基因表达，定位和交联到孢子皮中。
6. Temporal and spatial regulation of protein cross-linking by the pre-assembled substrates of a Bacillus subtilis spore coat transglutaminase [O] . Catarina G. Fernandes, Diogo Martins, Guillem Hernandez, 2019

机译：枯草芽孢杆菌孢子衣转谷氨酰胺酶的预组装底物对蛋白质交联的时空调节
7. Localization of the Transglutaminase Cross-Linking Sites in the Bacillus subtilis Spore Coat Protein GerQ▿ [O] . Monroe, Alicia, Setlow, Peter 2006

机译：枯草芽孢杆菌孢子外壳蛋白GerQ▿中转谷氨酰胺酶交联位点的定位

Reinforcement of Bacillus subtilis spores by cross-linking of outer coat proteins during maturation

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