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A novel gene, lstC, of Listeria monocytogenes is implicated in high salt tolerance

机译:李斯特菌的新基因lstC与高耐盐性有关

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摘要

Listeria monocytogenes, causative agent of human listeriosis, has been isolated from a wide variety of foods including deli meats, soft cheeses, cantaloupes, sprouts and canned mushrooms. Standard control measures for restricting microbial growth such as refrigeration and high salt are often inadequate as L. monocytogenes grows quite well in these environments. In an effort to better understand the genetic and physiological basis by which L. monocytogenes circumvents these controls, a transposon library of L. monocytogenes was screened for changes in their ability to grow in 7% NaCl and/ or at 5 ℃. This work identified a transposon insertion upstream of an operon, here named lstABC, that led to a reduction in growth in 7% NaCl. In-frame deletion studies identified lstC which codes for a GNAT-acetyltransferase being responsible for the phenotype. Transcriptomic and RT-PCR analyses identified nine genes that were upregulated in the presence of high salt in the ΔlstC mutant. Further analysis of lstC and the genes affected by ΔlstC is needed to understand LstC's role in salt tolerance.
机译:单核细胞增生李斯特菌是人类李斯特菌病的致病因子,已从多种食品中分离出来,包括熟食肉,软奶酪,哈密瓜,豆芽和蘑菇罐头。由于单核细胞增生李斯特氏菌在这些环境中生长良好,因此限制微生物生长的标准控制措施(例如冷藏和高盐)常常不足。为了更好地理解单核细胞增生李斯特氏菌避开这些对照的遗传和生理基础,筛选了单核细胞增生李斯特氏菌转座子文库,以了解它们在7%NaCl和/或5℃下生长的能力的变化。这项工作确定了一个转座子插入一个称为lstABC的操纵子的上游,导致7%NaCl的生长减少。框内删除研究确定了lstC,其编码负责该表型的GNAT-乙酰基转移酶。转录组学和RT-PCR分析鉴定了9个在ΔlstC突变体中存在高盐时上调的基因。需要进一步分析lstC和受ΔlstC影响的基因,以了解LstC在耐盐性中的作用。

著录项

  • 来源
    《Food microbiology》 |2015年第6期|72-82|共11页
  • 作者单位

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Listeria monocytogenes; Salt growth; Stress adaptation; Food preservation;

    机译:李斯特菌;盐分增长;压力适应;食品保鲜;

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