• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Campylobacter jejuni contamination of broiler carcasses: Population dynamics and genetic profiles at slaughterhouse level
【24h】

Campylobacter jejuni contamination of broiler carcasses: Population dynamics and genetic profiles at slaughterhouse level

机译：空肠弯曲杆菌对肉鸡屠体的污染：屠宰场的种群动态和遗传特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Six slaughter batches deriving from six typical industrial broiler flocks were examined for the presence, quantity and genetic characteristics of contaminating Campylobacter jejuni (C. jejuni) during various stages of slaughtering and carcass processing. To assess the contamination dynamics of the carcasses, the analyses were always conducted on neck-skin samples from the same pre-selected and carefully marked carcasses in each batch. The skin samples were taken sequentially at three successive slaughter-line locations in the evisceration room, after three-day refrigeration and after three-day freezing procedure. Caecal samples from the same animals were also tested, as well as samples from the slaughterhouse environment before and after slaughtering. The samples were analysed by the ISO10272 isolation method; campylobacters from neck-skin samples were also quantified. Isolates were species-identified and genotyped by pulsed-field gel electrophoresis (PFGE). On average, the highest C. jejuni skin contamination was detected at the first sampling point (post-plucking), suggesting that the majority of Campylobacter contamination actually occurs before the entrance to the eviscerating room, probably during the preceding plucking stage. In two out of five positive batches, an additional increase in contamination was recorded after the evisceration step. An evident trend of increasing contamination level was detected when successive batches were compared at each of two initial sampling sites in the evisceration room, indicating an accumulation of contaminating C. jejuni at some point before the evisceration room. Three-day refrigeration and three-day freezing caused a 4.5- and 142-fold drop in mean C. jejuni CFU counts, respectively. All pre-slaughtering samples from the slaughterhouse environment were negative and all post-slaughtering samples, except water from the scalding tank, were positive. Pulsotypes were limited: altogether five different types were detected, typically one type per batch. The PFGE results from the slaughterhouse environment isolates indicate that cross-contamination is possible (multiple pulsotypes detected in e.g. eviscerating machine). Nevertheless, this was not confirmed in carcasses: analyses of neck-skin isolates suggest that carcasses are contaminated by their own caecal/farm/flock pulsotype.
机译：检查了来自六个典型工业肉鸡群的六个屠宰批次在屠宰和car体加工各个阶段的污染空肠弯曲杆菌（C. jejuni）的存在，数量和遗传特征。为了评估屠体的污染动态，始终对每批相同预选且经过仔细标记的屠体的颈部皮肤样品进行分析。经过三天的冷藏和三天的冷冻程序后，依次在剔除室中的三个连续的屠宰线位置采集皮肤样本。还测试了来自相同动物的盲肠样品，以及在屠宰前后来自屠宰场环境的样品。样品通过ISO10272分离方法进行分析；还定量了来自颈部皮肤样品的弯曲杆菌。通过脉冲场凝胶电泳（PFGE）对分离物进行物种鉴定和基因分型。平均而言，在第一个采样点（拔毛后）检测到最高的空肠弯曲杆菌皮肤污染，这表明大多数弯曲杆菌污染实际上发生在进入内脏房之前，可能是在拔毛之前。在五分之二的阳性批次中，在内脏去除步骤后记录到污染进一步增加。当在剔除室中的两个初始采样点分别对连续批次进行比较时，发现了明显的污染水平上升趋势，这表明在剔除室之前的某个时间点，空肠弯曲菌的积累。三天冷藏和三天冷冻分别导致空肠弯曲杆菌CFU计数分别下降4.5和142倍。屠宰场环境中的所有屠宰前样品均为阴性，除烫伤池中的水外，所有屠宰后样品均为阳性。脉冲型是有限的：总共检测到五种不同类型，通常每批检测一种。屠宰场环境分离物的PFGE结果表明可能发生交叉污染（在例如内脏器中检测到多种脉冲型）。然而，尸体尚未得到证实：对颈部皮肤分离物的分析表明，尸体受到其自身盲肠/农场/羊群脉冲型的污染。

著录项

  • 来源
    《Food microbiology》 |2015年第9期|97-101|共5页
  • 作者

    Igor Gruntar; Majda Biasizzo; Darja Kusar; Mateja Pate; Matjaz Ocepek;

  • 作者单位

    University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Gerbiceva 60, 1000 Ljubljana, Slovenia;

    Veterinary Faculty, Institute of Food Hygiene and Bromatology, Gerbiceva 60, 1000 Ljubljana, Slovenia;

    Veterinary Faculty, Institute of Microbiology and Parasitology, Gerbiceva 60, 1000 Ljubljana, Slovenia;

    Veterinary Faculty, Institute of Microbiology and Parasitology, Gerbiceva 60, 1000 Ljubljana, Slovenia;

    Veterinary Faculty, Institute of Microbiology and Parasitology, Gerbiceva 60, 1000 Ljubljana, Slovenia;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Campylobacter jejuni; Carcasses contamination; Poultry processing; Slaughterhouse; Contamination reduction;

    机译：空肠弯曲菌;体污染;家禽加工;屠房减少污染;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号