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A proposed harmonized LPS molecular-subtyping scheme for Cronobacter species

机译:拟议的克氏杆菌属细菌LPS分子亚型协调方案

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摘要

Cronobacter are opportunistic pathogens, which cause infections in all age groups. To aid the characterization of Cronobacter in foods and environments a harmonized LPS identification scheme for molecular serotyping is needed. To this end, we studied 409 Cronobacter isolates representing the seven Cronobacter species using two previously reported molecular serotyping schemes, described here as Mullane-Jarvis (M-J) and Sun schemes. PCR analysis revealed many overlapping results that were obtained when independently applying the two serotyping schemes. There were complete agreements between the two PCR schemes for Cronobacter sakazakii (Csak) O:1, Csak O:3, and Csak O:7 serotypes. However, only thirty-five of 41 Csak O:4 strains, identified using the M-J scheme, were PCR-positive with the Sun scheme primers. Also the Sun scheme Csak O:5 primers failed to identify this serotype in any of the C. sakazakii strains tested, but did recognize seven Cronobacter turicensis strains, which were identified as Ctur O:3 using the M-J scheme. Similarly, the Sun scheme Csak 0:6 primers recognized 30 Cronobacter malonaticus O:2 strains identified with the M-J scheme, but failed to identify this serotype in any C. sakazakii strain investigated. In this report, these findings are summarized and a harmonized molecular-serotyping scheme is proposed which is predicated on the correct identification of Cronobacter species, prior to serotype determination. In summary, fourteen serotypes were identified using the combined protocol, which consists of Csak O:1-O:4, and Csak O:7; Cmal O:1-O:2; Cdub O:1-O:2, Cmuy O:1-O:2, Cuni O:1, as well as Ctur O:1 and Ctur O:3.
机译:克罗诺杆菌是机会病原体,可在所有年龄段引起感染。为了帮助表征食品和环境中的克罗诺杆菌,需要一种用于分子血清分型的统一的LPS鉴定方案。为此,我们使用两种先前报道的分子血清分型方案研究了代表7种克罗诺杆菌的409种克罗诺杆菌分离株,在此将其描述为Mullane-Jarvis(M-J)和Sun方案。 PCR分析显示,当独立应用两种血清分型方案时,获得了许多重叠的结果。阪崎肠杆菌(Csak)O:1,Csak O:3和Csak O:7血清型的两种PCR方案之间有完整的协议。但是,使用M-J方案鉴定的41个Csak O:4菌株中只有35株使用Sun方案引物进行PCR阳性。同样,Sun方案Csak O:5引物在任何测试的阪崎肠杆菌中均未鉴定出该血清型,但确实识别了7株土耳克氏杆菌,使用M-J方案鉴定为Ctur O:3。同样,Sun方案Csak 0:6引物可识别30株用M-J方案鉴定的丙酸克鲁氏杆菌O:2菌株,但未能在任何调查的阪崎肠杆菌中鉴定该血清型。在本报告中,对这些发现进行了总结,并提出了一种统一的分子血清分型方案,该方案基于血清型确定之前正确鉴定cronobacter菌种。总之,使用组合方案鉴定了14种血清型,包括Csak O:1-O:4和Csak O:7。 Cmal O:1-O:2; Cdub O:1-O:2,Cmuy O:1-O:2,Cuni O:1以及Ctur O:1和Ctur O:3。

著录项

  • 来源
    《Food microbiology》 |2015年第9期|38-43|共6页
  • 作者单位

    UCD Centre for Food Safety, WHO Collaborating Centre for Research, Reference, and Training on Cronobacter, University College Dublin, Belfield, Dublin 4, Ireland;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    Food Directorate, Bureau of Microbial Hazards/Health Canada, Ottawa, ON, Canada;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    CFSAN, FDA, Laurel, MD, USA;

    Mead Johnson Nutrition, Evansville, IN, USA;

    CFSAN, FDA College Park, MD, USA;

    College of Life Sciences, University of Dundee, Scotland, UK;

    Food Directorate, Bureau of Microbial Hazards/Health Canada, Ottawa, ON, Canada;

    UCD Centre for Food Safety, WHO Collaborating Centre for Research, Reference, and Training on Cronobacter, University College Dublin, Belfield, Dublin 4, Ireland;

    CFSAN, FDA, Laurel, MD, USA,3408, MOD 1 Facility, Virulence Mechanisms Branch, (HFS-025), Division of Virulence Assessment, OARSA, Center for Food Safety and Applied Nutrition, U. S. Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cronobacter; LPS; Molecular serotyping; SDS-PAGE;

    机译:克罗诺杆菌LPS;分子血清分型;SDS页面;

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