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Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from home made white brine cheese

机译:从自制白色盐水干酪中分离的非发酵肠球菌粪便菌株的致癌潜力和安全性评估

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摘要

Four LAB strains, isolated from Bulgarian home made white brine cheese, were selected for their effective inhibition against Listeria monocytogenes. According to their biochemical and physiological characteristics, the strains were classified as members of Enterococcus genus, and then identified as Enterococcus faecium by 16S rDNA sequencing. Their bacteriocin production and inhibitory spectrum were evaluated together with the occurrence of several bacteriocin genes (entA, enfB, entP, entL50B). Their virulence potential and safety was assessed both using PCR targeted to the genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and ode and by phenotypical tests for antibiotic resistance, gelatinase, lipase, DNAse and a- and β-haemolysis. The E. faecium strains harboured at least one enterocin gene while the occurrence of virulence, antibiotic resistance and biogenic amines genes was limited. Considering their strong antimicrobial activity against L monocytogenes strains, the four E. faecium strains exhibited promising potential as bio-preservatives cultures for fermented food productions.
机译:从保加利亚自制的白色盐水干酪中分离出四种LAB菌株,以有效抑制李斯特菌。根据其生化和生理特性,将菌株分类为肠球菌属成员,然后通过16S rDNA测序鉴定为粪便肠球菌。评估了它们的细菌素产生和抑制谱以及几种细菌素基因(entA,enfB,entP,entL50B)的出现。使用针对基因gelE,hyl,asa1,esp,cylA,efaA,ace,vanA,vanB,hdc1,hdc2,tdc和ode的PCR以及针对抗生素抗性,明胶酶,脂肪酶的表型测试,评估了它们的毒力潜力和安全性。 ,DNAse以及a和β溶血。粪肠球菌菌株具有至少一个肠球菌基因,而毒力,抗生素抗性和生物胺基因的发生受到限制。考虑到它们对单核细胞增生李斯特氏菌菌株的强抗微生物活性,四种粪肠球菌菌株作为发酵食品生产的生物防腐剂培养物显示出有希望的潜力。

著录项

  • 来源
    《Food microbiology》 |2014年第4期|228-239|共12页
  • 作者单位

    Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell'Universita 16, 35020 Legnaro, PD, Italy;

    Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell'Universita 16, 35020 Legnaro, PD, Italy;

    Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell'Universita 16, 35020 Legnaro, PD, Italy;

    UMR INRA Secalim 1014 ONIRIS, rue Geraudiere BP 82225, 44322 Nantes Cedex3, France;

    UMR INRA Secalim 1014 ONIRIS, rue Geraudiere BP 82225, 44322 Nantes Cedex3, France;

    Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 580, Bl. 14, 05508-000 Sao Paulo, SP, Brasil;

    Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 580, Bl. 14, 05508-000 Sao Paulo, SP, Brasil;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Enterococcus faecium; Bacteriocin; Anti-listeria monocytogenes activity; Safety; Virulence; White brine cheese;

    机译:粪肠球菌;细菌素抗单核细胞增生李斯特菌活性;安全;毒力;白盐水奶酪;

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