• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Diminution of 2,3,5-triphenyltetrazolium chloride toxicity on Listeria monocytogenes growth by iron source addition to the culture medium
【24h】

Diminution of 2,3,5-triphenyltetrazolium chloride toxicity on Listeria monocytogenes growth by iron source addition to the culture medium

机译：铁源添加到培养基中减少2,3,5-三苯基四唑氯化物对单核细胞增生李斯特菌生长的毒性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tetrazolium salts (TTZ) such as 2,3,5-triphenyltetrazolium chloride (TTC) are readily reduced by bacterial populations of various genus. The reduced form of these redox indicators is conspicuously colored allowing a quick and easy detection of growth. The studies are mainly confined to Gram negative bacteria because of an important toxic effect of tetrazolium salts on Gram positive bacteria. Indeed, we observed an important impact of different tetrazolium salts on Listeria monocytogenes growth, curiously limited to an increase in the duration of the lag phase. In this study, we demonstrate that increasing the iron concentration in a medium containing TTC leads to a significant decrease of the lag phase. L. monocytogenes growth was kinetically measured and growth parameters were estimated using the Baranyi model. While lag phase diminution was found to be iron concentration dependent, growth rate was not affected. Addition of iron enables growth of some strains totally inhibited by a 0.4 g/l of TTC and for the other a significant reduction of the latency is observed. The nature of the mechanism resulting in a decrease of the observed lag phase remains unclear. Then, the use of iron supplementation may be proposed to overcome the inhibitory effect of TTC on L monocytogenes.
机译：诸如2,3,5-三苯基四唑氯化物（TTC）之类的四唑盐（TTZ）容易因各种细菌属而减少。这些氧化还原指示剂的简化形式显色，可以快速，轻松地检测到生长。由于四唑盐对革兰氏阳性细菌具有重要的毒性作用，因此研究主要局限于革兰氏阴性细菌。确实，我们观察到了不同的四唑盐对单核细胞增生李斯特菌生长的重要影响，奇怪地是，这种作用仅限于延迟期的持续时间。在这项研究中，我们证明增加含TTC的培养基中铁的浓度会导致滞后相的显着降低。动力学测量单核细胞增生李斯特氏菌的生长，并使用Baranyi模型估算生长参数。虽然发现滞后相减少与铁浓度有关，但生长速度没有受到影响。铁的添加使得某些菌株的生长完全被0.4 g / l的TTC抑制，对于其他菌株，观察到等待时间的显着减少。导致观察到的滞后阶段减少的机制的性质尚不清楚。然后，可以建议使用铁补充剂来克服TTC对单核细胞增生李斯特氏菌的抑制作用。

著录项

  • 来源
    《Food microbiology》 |2014年第4期|1-5|共5页
  • 作者

    Thomas Junillon; Jean-Pierre Flandrois;

  • 作者单位

    Universite de Lyon 1, F-69000 Lyon, CNRS, UMR5558, Laboratoire de Biometrie et Biologie Evolutive, F-69622 Villeurbarnne, France,bioMerieux SA departement Microbiologie industrielle, Chemin de l'Orme 69280 Marcy l'Etoile, France;

    Universite de Lyon 1, F-69000 Lyon, CNRS, UMR5558, Laboratoire de Biometrie et Biologie Evolutive, F-69622 Villeurbarnne, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Triphenyltetrazolium chloride; Listeria monocytogenes; Iron source; Growth modeling;

    机译：氯化三苯四唑;李斯特菌;铁源;成长模型;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号