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Persistent Listeria monocytogenes subtypes isolated from a smoked fish processing facility included both phage susceptible and resistant isolates

机译：从熏鱼加工设施中分离出的持久性单核细胞增生李斯特菌亚型包括噬菌体易感和耐药菌

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摘要

Contamination of Ready-To-Eat foods with Listeria monocytogenes can typically be traced back to postprocessing contamination from environmental sources; contamination is often linked to subtypes that persist in food associated environments. Although phage-based biocontrol strategies have been proposed for controlling this pathogen, information on the efficacy of phage treatment against diverse L. monocytogenes subtypes from food associated environments is still limited. We identified subtypes that were repeatedly found ("persistent") in a smoked fish processing facility by using EcoRI ribotyping data for isolates obtained in 1998-2009. PFGE analysis of 141 isolates (9 ribotypes) supported persistence for up to 11 years. Characterization of selected isolates, representing persistent subtypes, against a panel of 28 listeriaphages showed a wide range of likelihood of phage susceptibility, ranging from 4.6% (for 7 ribotype DUP-1043A isolates) to 95.4% (for 7 ribotype DUP-1044A isolates). In challenge studies with 10~5 and 10~6 CFU/ml L. monocytogenes, using phage cocktails and a commercial phage product at different phage-host ratios, one isolate (ribotype DUP-1043A) was not affected by any treatment. A reduction in L monocytogenes counts of up to 4 log units was observed, after 8 h of treatment, in isolates of two ribotypes, but subsequent re-growth occurred. Survivor isolates obtained after 24 h of treatment showed decreased susceptibility to individual phages included in the phage cocktail, suggesting rapid emergence of resistant subtypes.
机译：即食食品被单核细胞增生性李斯特菌污染通常可以追溯到来自环境源的后处理污染。污染通常与在与食物相关的环境中持续存在的亚型有关。尽管已经提出了基于噬菌体的生物控制策略来控制这种病原体，但是关于从食物相关环境中针对多种单核细胞增生李斯特菌亚型的噬菌体治疗功效的信息仍然有限。通过使用1998-2009年获得的分离株的EcoRI核糖分型数据，我们确定了在熏制鱼加工设施中反复发现（“持久”）的亚型。对141个分离株（9个核糖型）的PFGE分析支持了长达11年的持久性。针对一组28个李斯特菌的代表持久性亚型的分离株的表征显示了广泛的噬菌体敏感性可能性，范围从4.6％（对于7个核糖型DUP-1043A分离株）到95.4％（对于7个核糖型DUP-1044A分离株） 。在以10〜5和10〜6 CFU / ml单核细胞增生李斯特氏菌进行的攻毒研究中，使用噬菌体混合物和商业噬菌体产品以不同噬菌体-宿主比例使用，一种分离物（核糖型DUP-1043A）不受任何处理的影响。在处理8小时后，在两种核糖型分离株中观察到单核细胞增生李斯特氏菌计数最多减少4个对数单位，但随后发生了重新生长。治疗24小时后获得的存活分离株显示出对噬菌体混合物中包括的单个噬菌体的敏感性降低，表明抗性亚型迅速出现。

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  • 来源
    《Food microbiology》 |2013年第1期|38-48|共11页
  • 作者

    Kitiya Vongkamjan; Sherry Roof; Matthew J. Stasiewicz; Martin Wiedmann;

  • 作者单位

    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA;

    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA;

    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA;

    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Listeria monocytogenes; Listeriaphage; Food processing facility;

    机译：李斯特菌;李斯特氏菌;食品加工设施;

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