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pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

机译：基于pilF多态性的实时PCR区分创伤弧菌菌株与人类健康的相关性

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摘要

The Gram-negative bacterium Vibrio vulnificus is a common inhabitant of estuarine environments. Globally, V. vulnificus is a significant foodborne pathogen capable of causing necrotizing wound infections and primary septicemia, and is a leading cause of seafood-related mortality. Unfortunately, molecular methods for the detection and enumeration of pathogenic V. vulnificus are hampered by the genetically diverse nature of this pathogen, the range of different biotypes capable of infecting humans and aquatic animals, and the fact that V. vulnificus contains pathogenic as well as non-pathogenic variants. Here we report an alternative approach utilizing the development of a real-time PCR assay for the detection of pathogenic V. vulnificus strains based on a polymorphism in pilF, a gene previously indicated to be associated with human pathogenicity. Compared to human serum reactivity, the real-time PCR assay successfully detected pathogenic strains in 46 out of 47 analysed V. vulnificus isolates (97.9%). The method is also rapid, sensitive, and more importantly can be reliably utilised on biotype 2 and 3 strains, unlike other current methods for V. vulnificus virulence differentiation.
机译：革兰氏阴性菌弧菌是河口环境的常见居民。在全球范围内，V。vulnificus是一种重要的食源性病原体，能够导致坏死性伤口感染和原发性败血病，并且是海鲜相关死亡率的主要原因。不幸的是，该病原体的遗传多样性，能够感染人类和水生动物的不同生物类型的范围以及该病原体以及病原体的事实阻碍了用于检测和枚举致病性V. vulnificus的分子方法。非致病性变体。在这里，我们报告了一种替代方法，该方法利用实时PCR检测技术的发展来检测基于pilF多态性的致病性V. vulnificus菌株，该基因先前已表明与人类致病性相关。与人血清反应性相比，实时荧光定量PCR技术成功检测出47种分析过的V. vulnificus分离株中的46种（97.9％）的致病菌。该方法还快速，灵敏，并且更重要的是可以可靠地用于2型和3型生物菌株，这与其他当前用于创伤弧菌毒力分化的方法不同。

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  • 来源
    《Food microbiology》 |2012年第1期|p.17-23|共7页
  • 作者

    Craig Baker-Austin; Elizabeth Lemm; Rachel Hartnell; James Lowther; Richard Onley; Carmen Amaro; James D. Oliver; David Lees;

  • 作者单位

    Centre for Environment Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset DT4 8UB, UK;

    University of Surrey, Cuildford, Surrey, UK;

    Centre for Environment Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset DT4 8UB, UK;

    Centre for Environment Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset DT4 8UB, UK;

    University of Surrey, Cuildford, Surrey, UK;

    Departamento de Microbiologia, Facultad de Biologia, Universidad de Valencia, Valencia, Spain;

    Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina, USA;

    Centre for Environment Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset DT4 8UB, UK;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    pathogen; V. vulnificus; pilF; real-time PCR; detection; oyster;

    机译：病原;V. vulnificus;pilF;实时PCR;检测;牡蛎;

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