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首页> 外文期刊>Food microbiology >Development of a multilocus variable number of tandem repeat typing method for Oenococcus oeni
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Development of a multilocus variable number of tandem repeat typing method for Oenococcus oeni

机译：Oenococcus oeni多座位可变数目串联重复键入法的开发

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摘要

Oenococcus oeni is responsible for the malolactic fermentation of wine. Genomic diversity has already been established in this species. In addition, winemakers usually report varying starter culture efficiency. The monitoring of indigenous and selected strains is essential for understanding strain survival and implantation during the winemaking process. In this study, we report the development of the first typing scheme for 0. oeni using multiple-locus variable number of tandem repeat analysis (VNTR). The discriminatory power of 14 out of 44 tandem repeat loci in the genome of the PSU-1 strain was initially evaluated with a test collection of 18 genotypically distinct starter strains. Then five VNTR loci, which can be easily scored with the technology used here, were identified and used to genotype a collection of 236 strains, previously classified by restriction endonuclease analysis-pulsed-field gel electrophoresis (REA-PFGE) and multilocus sequence typing (MLST) into 136 REA-PFGE types or 110 MLST types. The discriminatory power of VNTR (as determined by Simpson's index of discrimination) was higher than that of the other two methods, with 201 VNTR types. The targeted VNTR markers were found to be stable and did not change for the clones of the same strain deposited in a collection at intervals of several years. Strains isolated from the different wine producing areas or the products were assigned to phylogenetic groups and were statistically linked with the VNTR profiles. Another interesting observation was that the loci were found in sequences homologous to regions encoding for membrane-anchored proteins.
机译：Oenococcus oeni负责葡萄酒的苹果酸发酵。该物种已经建立了基因组多样性。此外，酿酒师通常会报告不同的起子文化效率。监测本地和选定的菌株对于了解酿酒过程中菌株的存活和植入至关重要。在这项研究中，我们报告了使用多位点可变数目的串联重复分析（VNTR）的0. oeni的第一个分型方案的发展。最初使用18种基因型不同的起始菌株的测试集合初步评估了PSU-1菌株基因组中44个串联重复基因座中的14个的鉴别能力。然后，鉴定出五个VNTR基因座，这些基因可以很容易地用此处使用的技术进行评分，并用于对236个菌株进行基因分型，这些菌株先前通过限制性内切酶分析脉冲场凝胶电泳（REA-PFGE）和多基因座序列分型进行了分类（ MLST）分为136种REA-PFGE类型或110种MLST类型。 VNTR的区分能力（由辛普森的区分指数确定）高于其他两种方法，具有201种VNTR类型。发现靶向的VNTR标记物是稳定的，并且对于以几年的间隔沉积在集合中的同一菌株的克隆没有变化。从不同的葡萄酒产区或产品中分离出的菌株被分配到系统发育组，并与VNTR谱进行统计学关联。另一个有趣的发现是在与编码膜锚定蛋白的区域同源的序列中发现了基因座。

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  • 来源
    《Food microbiology》 |2012年第2期|p.340-347|共8页
  • 作者

    Olivier Claisse; Aline Lonvaud-Funel;

  • 作者单位

    Univ. Bordeaux, ISW, EA 4577 Enologie, F-33140 Villenave d'Omon, France,INRA, ISW, USC 1219 Enologie, F-33140 Villenave d'Ornon, France;

    Univ. Bordeaux, ISW, EA 4577 Enologie, F-33140 Villenave d'Omon, France,INRA, ISW, USC 1219 Enologie, F-33140 Villenave d'Ornon, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    oenococcus oeni; VNTR; membrane-anchored protein;

    机译：葡萄球菌VNTR;膜锚蛋白;

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