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Comparison of different sample preparation procedures for the detection and isolation of Escherichia coli O157:H7 and Non-0157 STECs from leafy greens and cilantro

机译:比较从绿叶蔬菜和香菜中检测和分离大肠杆菌O157:H7和Non-0157 STEC的不同样品制备程序

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摘要

The FDA Bacteriological Analytical Manual (BAM) method for the detection/isolation of Shiga toxin-producing Escherichia coli (STEC) involves enrichment of produce rinses, blended homogenates or stomached homogenates. However, the effectiveness of rinsing produce to remove attached bacteria is largely unknown. Moreover, PCR inhibitors can be released under physical treatment. The study objective was to determine the relative effectiveness of recovery methods for STEC contaminated produce. Spinach, lettuce, and cilantro were contaminated with E. coli O157:H7 or a non-0157 STEC, subjected to both the BAM method and a soak method, and tested by real-time PCR and cultural methods. For O157:H7 and non-O157:H7 STECs, the soak method was significantly more productive than leafy green rinses. Of 320 test portions, PCR of recovered colonies confirmed 148 were positive by rinsing and 271 were positive by soaking (an 83% increase in sensitivity). For recovery of O157:H7 from cilantro, of 60 test portions, positives were 38 by soaking, 41 by stomaching, and 28 by blending. Soaking and stomaching were significantly more productive than blending, although soaking was only arithmetically superior to stomaching. Based upon these results, it is recommended that a soak method replace the current BAM procedures.
机译:FDA细菌分析手册(BAM)方法用于检测/分离产生志贺毒素的大肠杆菌(STEC),涉及产品漂洗液,混合匀浆或胃匀浆的富集。但是,漂洗产品去除附着细菌的效果很大程度上未知。而且,PCR抑制剂可以在物理处理下释放。研究目的是确定针对STEC污染产品的回收方法的相对有效性。菠菜,生菜和香菜被大肠杆菌O157:H7或非0157 STEC污染,并同时采用BAM方法和浸泡方法,并通过实时PCR和培养方法进行测试。对于O157:H7和非O157:H7 STEC,浸泡方法比绿叶绿色漂洗液生产率更高。在320个测试部分中,回收的菌落的PCR证实,漂洗后的阳性率为148,浸泡后的阳性为271(灵敏度提高了83%)。为了从香菜中回收O157:H7,共分60个测试部分,浸泡时阳性为38,胃stomach为41,混合时为28。尽管在算术上仅比在混合条件下浸泡,但浸泡和混合过程比混合混合的生产率更高。根据这些结果,建议采用浸泡方法代替当前的BAM程序。

著录项

  • 来源
    《Food microbiology》 |2012年第2期|p.423-426|共4页
  • 作者单位

    Division of Microbiology, Microbial Methods Development Branch, Office of Regulatory Sciences, Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, 5100 Paint Branch Parkway, Rm 3E-023, HFS - 711 College Park, MD 20740, USA;

    Oak Ridge Research Institute for Science and Education, PO Box 117, Oak Ridge, TN 37830, USA;

    Division of Microbiology, Microbial Methods Development Branch, Office of Regulatory Sciences, Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, 5100 Paint Branch Parkway, Rm 3E-023, HFS - 711 College Park, MD 20740, USA;

    Division of Microbiology, Microbial Methods Development Branch, Office of Regulatory Sciences, Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, 5100 Paint Branch Parkway, Rm 3E-023, HFS - 711 College Park, MD 20740, USA;

    Oak Ridge Research Institute for Science and Education, PO Box 117, Oak Ridge, TN 37830, USA;

    Oak Ridge Research Institute for Science and Education, PO Box 117, Oak Ridge, TN 37830, USA;

    Division of Microbiology, Microbial Methods Development Branch, Office of Regulatory Sciences, Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, 5100 Paint Branch Parkway, Rm 3E-023, HFS - 711 College Park, MD 20740, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    methodology; STEC; raw produce;

    机译:方法论;STEC;原料;

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