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首页> 外文期刊>Food microbiology >Molecular identification and pectate lyase production by Bacillus strains involved in cocoa fermentation
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Molecular identification and pectate lyase production by Bacillus strains involved in cocoa fermentation

机译：参与可可发酵的芽孢杆菌菌株的分子鉴定和果胶酸裂解酶的产生

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摘要

We have previously reported the implication of Bacillus in the production of pectinolytic enzymes during cocoa fermentation. The objective of this work was to identify the Bacillus strains isolated from cocoa fermentation and study their ability to produce pectate lyase (PL) in various growth conditions. Ninety-eight strains were analyzed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Four different banding patterns were obtained leading to the clustering of the bacterial isolates into 4 distinct ARDRA groups. A subset of representative isolates for each group was identified by 16S rRNA gene partial sequencing. Six species were identified: Bacillus subtilis, Bacillus pumilus, Bacillus sphaericus. Bacillus cereus. Bacillus thuringiensis, together with Bacillus fiisiformis which was isolated for the first time from cocoa fermentation. The best PL producers, yielding at least 9 U/mg of bacterial dry weight, belonged to B. fiisiformis, B. subtilis, and B. pumilus species while those belonging to B. sphaericus, B. cereus and B. thuringiensis generally showed a low level of activity. Two kinds of PL were produced, as revealed by isoelectrofocusing: one with a pi of 9.8 produced by B. subtilis and B. fiisiformis, the other with a pi of 10.5 was produced by B. pumilus. Strains yielded about 2 fold more PL in a pectic compound medium than in glucose medium and maximum enzyme production occurred in the late stationary bacterial growth phase. Together all these results indicate that PL production in the bacilli studied is modulated by the growth phase and by the carbon source present in the medium.
机译：我们以前曾报道过可可发酵过程中芽孢杆菌对果胶分解酶产生的影响。这项工作的目的是鉴定从可可发酵中分离的芽孢杆菌菌株，并研究其在各种生长条件下产生果胶酸裂合酶（PL）的能力。通过扩增的核糖体DNA限制分析（ARDRA）分析了98个菌株。获得了四种不同的谱带模式，从而将细菌分离物聚集成4个不​​同的ARDRA组。通过16S rRNA基因部分测序鉴定每组代表性分离株的子集。确定了六个物种：枯草芽孢杆菌，短小芽孢杆菌，球形芽孢杆菌。蜡状芽孢杆菌。苏云金芽孢杆菌，以及首次从可可发酵中分离的费西芽孢杆菌。最好的PL生产者，其细菌干重至少为9 U / mg，属于B. fiisiformis，B.subtilis和B.pumilus种，而属于B. sphaericus，B。cereus和B. thuringiensis的种一般显示活动水平低。等电聚焦表明，产生了两种PL：一种是枯草芽孢杆菌和费氏芽孢杆菌产生的pi值为9.8，另一种是短小芽孢杆菌产生的pi值为10.5。菌株在果胶化合物培养基中产生的PL比在葡萄糖培养基中产生的PL多出约2倍，并且最大的酶产生发生在静止细菌的晚期生长阶段。所有这些结果共同表明，所研究的细菌的PL产量受生长期和培养基中碳源的调节。

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  • 来源
    《Food microbiology》 |2011年第1期|p.1-8|共8页
  • 作者

    Honore G. Ouattara; Sylvie Reverchon; Sebastien L. Niamke; William Nasser;

  • 作者单位

    Laboratoire de Biotechnologies, UFR Bisociences, Universite Cocody/Abidjan, 22 bp 582 Abidjan, Cote d'Ivoire,CNRS UMR5240 Microbiologie, Adaptation et Pathogenie (MAP), Universite Lyon 1, F-69622, INSA de Lyon, F-69621 Villeurbanne, France;

    rnCNRS UMR5240 Microbiologie, Adaptation et Pathogenie (MAP), Universite Lyon 1, F-69622, INSA de Lyon, F-69621 Villeurbanne, France;

    rnLaboratoire de Biotechnologies, UFR Bisociences, Universite Cocody/Abidjan, 22 bp 582 Abidjan, Cote d'Ivoire;

    rnCNRS UMR5240 Microbiologie, Adaptation et Pathogenie (MAP), Universite Lyon 1, F-69622, INSA de Lyon, F-69621 Villeurbanne, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacillus; cocoa fermentation; molecular identification; pectate lyase production;

    机译：芽孢杆菌可可发酵分子鉴定果胶酸裂解酶生产;

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