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首页> 外文期刊>Food microbiology >Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina
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Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina

机译：从阿根廷圣路易斯市肉类食品中分离的耶尔森菌小肠结肠炎耶尔森氏菌的脉冲场，PCR核糖分型和多重PCR分析

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摘要

The characterization of phenotypic and genotypic virulence markers of Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five V. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of virF, ail, ystA, and myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for ystB gene. Four Y. enterocolitica 2/0:9 (bio/serotype) and two 3/0:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFCE and PCR ribotyping, respectively. In both cases the Y. enterocolitica 2/0:9 strains were separately clustered from 1A and 3/0:5 strains. Meat foods might be vehicles of transmission of pathogenic Y. enterocolitica strains in our region.
机译：在阿根廷圣路易斯市主要从食品中分离出的属于生物型（B）1A，2和3的小肠结肠炎耶尔森氏菌菌株的表型和基因型毒力标记物的表征，并通过PFGE和PCR核型分析评估了它们的基因型多样性。我们的实验室第一次。对我们实验室中分离的35株肠球菌，2株参考菌株和33株菌株进行了研究。通过多重PCR研究了virF，ail，ystA和myfA基因的存在。通过简单的PCR研究了B1A菌株的致病潜力，B1A菌株是从我们地区的肉中分离出的肠球菌耶尔森氏菌的最主要生物型。四个B1A菌株的ystB基因呈阳性。在我们实验室中分离出的4株小肠结肠炎耶尔森菌2/0：9（生物/血清型）和2株3/0：5菌株在表型测试和多重PCR中显示了与毒力相关的结果。对于大多数菌株，毒力标志物的表达与其相应的基因型之间存在良好的相关性。通过PFCE和PCR核型分析分别产生了16个基因组类型（GT）和9个不同的基因间隔区（SR）组。在两种情况下，小肠结肠炎耶尔森氏菌2/0：9菌株分别与1A和3/0：5菌株聚类。肉类食品可能是我们地区致病性小肠结肠炎耶尔森氏菌菌株的传播媒介。

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  • 来源
    《Food microbiology》 |2011年第1期|p.21-28|共8页
  • 作者

    Cecilia S.M. Lucero Estrada; Lidia del Carmen Velazquez; Maria Esther Escudero; Gabriela Isabel Favier; Valeria Lazarte; Ana Maria Stefanini de Guzman;

  • 作者单位

    Microbiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

    rnMicrobiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

    rnMicrobiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

    rnMicrobiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

    rnMicrobiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

    rnMicrobiologia General, Area Microbiologia, Facultad de Quimica. Bioquimka y Farmacia, Universidad National de San Luis, Ejertito de los Andes 950 Bloque 1,San Luis 5700, Argentina;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    yersinia enterocolitica; molecular characterization; multiplex PCR; PFGE; PCR ribotyping;

    机译：小肠结肠炎耶尔森菌分子表征多重PCR PFGE;PCR核糖分型;

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