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Amino acids improve acid tolerance and internal pH maintenance in Bacillus cereus ATCC14579 strain

机译：氨基酸提高蜡样芽孢杆菌ATCC14579菌株的耐酸性和内部pH维持

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摘要

This study investigated the involvement of glutamate-, arginine- and lysine-dependent systems in the Acid Tolerance Response (ATR) of Bacillus cereus ATCC14579 strain. Cells were grown in a chemostat at external pH (pH_e) 7.0 and 5.5. Population reduction after acid shock at pH 4.0 was strongly limited in cells grown at pH 5.5 (acid-adapted) compared with cells grown at pH 7.0 (unadapted), indicating that B. cereus cells grown at low pH_e were able to induce a marked ATR. Glutamate, arginine and lysine enhanced the resistance of unadapted cells to pH 4.0 acid shock of 1-log or 2-log populations, respectively. Amino acids had no detectable effect on acid resistance in acid-adapted cells. An acid shock at pH 4.0 resulted in a marked drop in internal pH (pHj) in unadapted cells compared with acid-adapted cells. When acid shock was achieved in the presence of glutamate, arginine or lysine, pHj was maintained at higher values (6.31, 6.69 or 6.99, respectively) compared with pHj in the absence of amino acids (4.88). Acid-adapted cells maintained their pHj at around 6.4 whatever the condition. Agmatine (a competitive inhibitor of arginine decarboxylase) had a negative effect on the ability of B. cereus cells to survive and maintain their pHj during acid shock. Our data demonstrate that B. cereus is able to induce an ATR during growth at low pH. This adaptation depends on pH_i homeostasis and is enhanced in the presence of glutamate, arginine and lysine. Hence evaluations of the pathogenicity of B. cereus must take into account its ability to adapt to acid stress.
机译：这项研究调查了谷氨酸，精氨酸和赖氨酸依赖系统参与蜡样芽孢杆菌ATCC14579菌株的耐酸反应（ATR）。细胞在外部pH（pH_e）7.0和5.5的化学恒温器中生长。与在pH 7.0（未适应）下生长的细胞相比，在pH 5.5（经酸适应）下生长的细胞中pH 4.0酸冲击后的种群减少受到严格限制，这表明在低pH_e下生长的蜡状芽孢杆菌细胞能够诱导显着的ATR 。谷氨酸，精氨酸和赖氨酸分别增强了不适应细胞对1-log或2-log群体的pH 4.0酸冲击的抗性。氨基酸对酸适应细胞的抗酸性没有可检测的影响。与酸适应性细胞相比，pH 4.0的酸冲击导致未适应细胞的内部pH（pHj）明显下降。当在谷氨酸，精氨酸或赖氨酸存在下实现酸休克时，与不存在氨基酸的情况下pHj相比，pHj保持较高的值（分别为6.31、6.69或6.99）（4.88）。无论条件如何，适应酸的细胞的pHj均保持在6.4左右。胍丁胺（精氨酸脱羧酶的竞争性抑制剂）对蜡状芽孢杆菌细胞在酸休克期间存活和维持其pHj的能力具有负面影响。我们的数据表明蜡状芽孢杆菌能够在低pH下生长期间诱导ATR。这种适应取决于pH_i稳态，并且在存在谷氨酸，精氨酸和赖氨酸的情况下增强。因此，对蜡状芽孢杆菌致病性的评估必须考虑其对酸胁迫的适应能力。

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  • 来源
    《Food microbiology》 |2011年第3期|p.364-372|共9页
  • 作者

    Khadidja Senouci-Rezkallah; Philippe Schmitt; Michel P. Jobin;

  • 作者单位

    INRA, UMR408 Securite et Qualite des Produits d'Origine Vegetate, F-84914 Avignon, France;

    INRA, UMR408 Securite et Qualite des Produits d'Origine Vegetate, F-84914 Avignon, France,Universite d'Avignon et des Pays de Vaucluse, IUT, Site Agroparc, F-84911 Avignon, France;

    INRA, UMR408 Securite et Qualite des Produits d'Origine Vegetate, F-84914 Avignon, France,Universite d'Avignon et des Pays de Vaucluse, IUT, Site Agroparc, F-84911 Avignon, France,INRA, Universite d'Avignon et des Pays de Vaucluse,UMR408, F-84000 Avignon, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacillus cereus; chemostat; ATR; stress; PH_i; glutamate; arginine and lysine decarboxylase; arginine deiminase;

    机译：蜡状芽孢杆菌恒化器ATR;强调;PH_i;谷氨酸精氨酸和赖氨酸脱羧酶;精氨酸脱亚氨酶;

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