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PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes

机译：PCR ITS-RFLP：一种在葡萄上鉴定丝状真菌分离物的有用方法

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摘要

Restriction digestion analysis of the ITS products was tested as an easy method to identify isolates of filamentous fungi on grapes. Endonucleases Sdul, Hinfl, Msel, Haelll were used. Endonucleases Bftnl, Cfr9, Hpy1881, Maell or PspGl were used as necessary to complete discrimination. The 43 species studied generated 42 different composite profiles. Only the species P. thomii and P. glabrum gave the same composite profile. 96.3% strains tested could be differentiated to the species level with only four enzymes. Hundred ninety nine strains of filamentous fungi were isolated from various vineyards in Burgundy and identified by this method. Penicillium (58.5%) was the genus the most frequently isolated and no strains of the genus Aspergillus was isolated. P. spinolusum was the most isolated species of Penicillium (22.70%). The species C. cladiosporioides, B. cinerea, E. nigrum, A. alternata, T. koningiopsis, P. diplodiella, C. herbarum, A. alternatum, T. cucumeris and F. oxysporum were also isolated. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. This is the first exhaustive study of fungal diversity at species level in vineyard.
机译：测试ITS产品的限制性消化分析是鉴定葡萄丝状真菌分离物的简便方法。使用了内切核酸酶Sdul，Hinfl，Msel，Haelll。根据需要使用核酸内切酶Bftn1，Cfr9，Hpy1881，Maell或PspG1来完成区分。研究的43个物种产生了42种不同的复合特征。只有P. thomii和P. glabrum种具有相同的复合特征。仅用4种酶就可以将96.3％的菌株区分到种水平。从勃艮第的各个葡萄园中分离出一百九十九株丝状真菌，并通过这种方法进行鉴定。青霉菌（58.5％）是最常分离的属，没有分离出曲霉属的菌株。刺青霉是青霉菌中最分离的物种（22.70％）。还分离了C. cladiosporioides，B。cinerea，E。nigrum，A。alternata，T。koningiopsis，P。diplodiella，C.herbarum，A。alternatum，T。cucumeris和F. oxysporum。该技术是一种快速可靠的方法，适合常规鉴定丝状真菌。这可用于在短时间内从各种环境中筛选大量分离株。这是首次在葡萄园中对真菌多样性进行详尽的研究。

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  • 来源
    《Food microbiology》 |2011年第6期|p.1145-1154|共10页
  • 作者

    C.F. Diguta; B. Vincent; M. Guilloux-Benatier; H. Alexandra; S. Rousseaux;

  • 作者单位

    Laboratoire REW, Institut Universitaire de la Vigne et du Vin, Jules Cuyot, Universite de Bourgogne, BP 27877, 21078 Dijon Cedex, France;

    lnstitut Francois de la Vigne et du Vin, 21200 Beaune, France;

    Laboratoire REW, Institut Universitaire de la Vigne et du Vin, Jules Cuyot, Universite de Bourgogne, BP 27877, 21078 Dijon Cedex, France;

    Laboratoire REW, Institut Universitaire de la Vigne et du Vin, Jules Cuyot, Universite de Bourgogne, BP 27877, 21078 Dijon Cedex, France;

    Laboratoire REW, Institut Universitaire de la Vigne et du Vin, Jules Cuyot, Universite de Bourgogne, BP 27877, 21078 Dijon Cedex, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    filamentous fungi; pcr its-rflp; identification; crapes;

    机译：丝状真菌;pcr-rflp;鉴定;皱纹;

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