• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Yeast biodiversity from oleic ecosystems: Study of their biotechnological properties
【24h】

Yeast biodiversity from oleic ecosystems: Study of their biotechnological properties

机译：油酸生态系统中的酵母生物多样性：其生物技术特性的研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to know the yeast biodiversity from fresh olive (Olea europaea L.) fruits, olive paste (crush olives) and olive pomace (solid waste) from Arbequina and Cornicabra varieties. Yeasts were isolated from fruits randomly harvested at various olive groves in the region of Castilla La Mancha (Spain). Olive paste and pomace, a byproduct of the processing of this raw material, were also collected in sterile flasks from different oil mills. Molecular identification methodology used included comparison of polymerase chain reaction (PCR) amplicons of their 5.8S rRNA gene and internal transcribed spacers ITS1 and ITS2 followed by restriction pattern analysis (RFLP). For some species, sequence analysis of the 5.8S rDNA gene was necessary. The results were compared to sequences held in public databases (BLAST). These techniques allowed to identify fourteen different species of yeasts, belonging to seven different genera (Zygosaccharomyces, Pichia, Lachancea, Kluyveromyces, Saccharomyces, Candida, Torulaspora) from the 108 yeast isolates. Species diversity was thus considerable: Pichia caribbica, Zygosaccharomyces fer-mentati (Lachancea fermentati) and Pichia holstii (Nakazawaea holstii) were the most commonly isolated species, followed by Pichia mississippiensis, Lachancea sp., Kluyveromyces thermotolerans and Saccharomyces rosinii. The biotechnological properties of these isolates, was also studied. For this purpose, the activity of various enzymes (β-glucosidase, β-glucanase, carboxymethylcellulase, polygalacturonase, peroxidase and lipase) was evaluated. It was important that none of species showed lipase activity, a few had cellulase and polygalacturonase activities and the majority of them presented β-glucanase, β-glucosidase and peroxidase activities.
机译：这项研究的目的是了解来自Arbequina和Cornicabra品种的新鲜橄榄（Olea europaea L.）水果，橄榄糊（压榨橄榄）和橄榄果渣（固体废物）的酵母生物多样性。从卡斯蒂利亚拉曼恰（西班牙）地区各个橄榄树林中随机收获的果实中分离出酵母。橄榄糊和果渣（这种原料加工的副产品）也被收集在来自不同油厂的无菌烧瓶中。使用的分子鉴定方法包括比较其5.8S rRNA基因的聚合酶链反应（PCR）扩增子和内部转录的间隔子ITS1和ITS2，然后进行限制性酶切模式分析（RFLP）。对于某些物种，需要对5.8S rDNA基因进行序列分析。将结果与公共数据库（BLAST）中保存的序列进行比较。这些技术可以从108种酵母菌中鉴定出14种不同的酵母菌，它们属于7个不同的属（合子酵母，毕赤酵母，腊肠菜，克鲁维酵母菌，酿酒酵母，念珠菌，托鲁氏酵母）。因此，物种的多样性非常可观：最普遍分离的物种有加勒比毕赤酵母，发酵单胞菌（Lachancea fermentati）和霍尔斯蒂毕赤酵母（Nakazawaea holstii），其次是密西西比毕赤酵母，Lachancea sp。，耐热克鲁维酵母和酿酒酵母。还研究了这些分离物的生物技术特性。为此，评估了各种酶（β-葡糖苷酶，β-葡聚糖酶，羧甲基纤维素酶，聚半乳糖醛酸酶，过氧化物酶和脂肪酶）的活性。重要的是，没有一个物种表现出脂肪酶活性，少数物种具有纤维素酶和聚半乳糖醛酸酶活性，而大多数物种具有β-葡聚糖酶，β-葡萄糖苷酶和过氧化物酶活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号