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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Identification and characterization of yeasts isolated from oleic ecosystems
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Identification and characterization of yeasts isolated from oleic ecosystems

机译：从油酸生态系统中分离的酵母鉴定与表征

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摘要

The population dynamics of yeasts in oleic ecosystems in the Castilla la Mancha region (Spain) was analysed for two consecutive years (2007 to 2008) in two different varieties (Cornicabra and Arbequina respectively). Yeasts were isolated from of fresh olives (Olea europaea L.) fruits, olive pomace (solid waste) and olive paste (crush olives). All yeast species were identified by RFLPs of their rDNA but in some species sequence analysis of the 5.8S rDNA gene was necessary. This study allowed to identify 108 yeast isolated which belonging to seven different genera (Zygosaccharomyces, Pichia, Lachancea, Kluyveromyces, Saccharomyces, Candida, Torulaspora) and fourteen species. The most representative species were Pichia caribbica, Zygosaccharomyces fermentati and Pichia holstii. The yeast characterization was studied by means of several enzymes how β-glucosidase, β-glucanase, carboxymethylcellulase, polygalacturonase, peroxydase and lipase and was observed that the mayority of them presented β-glucanase, β-glucosidase and peroxidase activities, a few had cellulase and polygalacturonase activities and none of species showed lipase activity.
机译：连续两年（Cornicabra和Arbequina）分析了Castilla La Mancha地区（西班牙）在Castilla La Mancha Region（西班牙）中的酵母酵母种群动态。酵母被隔离从新鲜橄榄（Olea Europaea L.）水果，橄榄渣（固体废物）和橄榄酱（Crush Olives）。所有酵母物种都是通过其RDNA的RFLP鉴定，但在某些物种中，需要5.8S rDNA基因的序列分析。该研究允许鉴定属于七种不同的属（Zygosaccharomyces，Pichia，Lachancea，Kluyveromyces，酿酒酵母，念珠菌，Torulaspora）和十四种物种的108酵eSteast。最具代表性的物种是Pichia Caribbica，Zygosaccharomyces fermenti和Pichia holstii。通过几种酶研究β-葡萄糖苷酶，β-葡聚糖酶，羧甲基纤维素酶，多糖酶，过氧化物酶和脂肪酶，并观察到它们呈现β-葡聚糖酶，β-葡糖苷酶和过氧化物酶活性的纤维素酶的含有纤维素酶和多肢乳糖酶活性，但没有种类没有显示脂肪酶活性。

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