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首页> 外文期刊>Food microbiology >Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment
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Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment

机译：高压二氧化碳处理诱导的大肠杆菌，单核细胞增生性李斯特菌和酿酒酵母的膜通透性和细胞死亡

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摘要

In this study, the relationship between (irreversible) membrane permeabilization and loss of viability in Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae cells subjected to high pressure carbon dioxide (HPCD) treatment at different process conditions including temperature (35-45 ℃), pressure (10.5-21.0 MPa) and treatment time (0-60 min) was examined. Loss of membrane integrity was measured as increased uptake of the fluorescent dye propidium iodide (PI) with spectrofluorometry, while cell inactivation was determined by viable cell count. Uptake of PI by all three strains indicated that membrane damage is involved in the mechanism of HPCD inactivation of vegetative cells. The extent of membrane permeabilization and cellular death increased with the severity of the HPCD treatment. The resistance of the three tested organisms to HPCD treatment changed as a function of treatment time, leading to significant tailing in the survival curves, and was dependent on pressure and temperature. The results in this study also indicated a HPCD-induced damage on nucleic acids during cell inactivation. Transmission electron microscopy showed that HPCD treatment had a profound effect on the intracel-lular organization of the micro-organisms and influenced the permeability of the bacterial cells by introducing pores in the cell wall.
机译：在这项研究中，在不可逆的条件下，膜的通透性与大肠杆菌，单核细胞增生李斯特菌和酿酒酵母细胞在不同温度，温度（35-45℃），压力等条件下的活力丧失之间的关系。检查（10.5-21.0 MPa）和处理时间（0-60分钟）。通过荧光分光光度法测量荧光染料碘化丙啶（PI）的摄取增加，从而测量膜完整性的损失，而通过活细胞计数确定细胞失活。所有三种菌株对PI的摄取表明，膜损伤与营养细胞HPCD失活的机制有关。膜通透性和细胞死亡的程度随HPCD治疗的严重程度而增加。三种被测生物对HPCD处理的抗性随处理时间的变化而变化，导致存活曲线明显拖尾，并取决于压力和温度。这项研究的结果还表明，HPCD诱导的细胞失活期间对核酸的破坏。透射电子显微镜显示，HPCD处理对微生物的胞内组织有深远的影响，并通过在细胞壁上引入孔来影响细菌细胞的通透性。

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  • 来源
    《Food microbiology》 |2010年第4期|p.541-549|共9页
  • 作者

    L. Garcia-Gonzalez; A.H. Geeraerd; J. Mast; Y. Briers; K. Elst; L. Van Ginneken; J.F. Van Impe; F. Devlieghere;

  • 作者单位

    Business Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), B-2400 Mol, Belgium Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Food2Know, Ghent University, B-9000 Ghent, Belgium;

    Department of Biosystems (BIOSYST/MeBioS) and Leuven Center for Bio-Science, Bio-Engineering and Bio-Technology (BioSCENTre), Katholieke Universiteit Leuven, B-3001 Leuven, Belgium;

    Department of Biocontrol, Veterinary and Agrochemical Research Centre (VAR), B-1180 Ukkel, Belgium;

    Department of Biosystems, Laboratory of Gene Technology, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium;

    Business Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), B-2400 Mol, Belgium;

    Business Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), B-2400 Mol, Belgium;

    Department of Chemical Engineering, Chemical and Biochemical Process Technology and Control (BioTeC), Katholieke Universiteit Leuven, B-3001 Leuven, Belgium;

    Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Food2Know, Ghent University, B-9000 Ghent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    high pressure carbon dioxide; membrane permeabilization; propidium iodide; escherichia coli; listeria monocytogenes; saccharomyces cerevisiae;

    机译：高压二氧化碳膜通透性碘化丙啶大肠杆菌;李斯特菌;酿酒酵母;

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