• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Effect of media, additives, and incubation conditions on the recovery of high pressure and heat-injured Clostridium botulinum spores
【24h】

Effect of media, additives, and incubation conditions on the recovery of high pressure and heat-injured Clostridium botulinum spores

机译：培养基，添加剂和培养条件对高压和热损伤肉毒梭菌孢子回收的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of additives and post-treatment incubation conditions on the recovery of high pressure and heat-injured (i.e., processed at 620 MPa and 95 and 100℃ for 5 min) spores of Clostridium botulinum strains, 62-A (proteolytic type A) and 17-B (nonproteolytic type B) was studied. High pressure and heat-injured spores were inoculated into TPGY (Trypticase-Peptone-Glucose-Yeast extract) anaerobic broth media containing additives (lysozyme, L-alanine, L-aspartic acid, dipicolonic acid, sodium bicarbonate, and sodium lactate) at various concentrations (0-10 μg/ml) individually or in combination. The spore counts of high pressure and heat-injured 62-A and 17-B recovered from TPGY broth containing lysozyme (10 μg/ml) incubated for 4 months versus that recovered from peptone-yeast extract-glucose-starch (PYGS) plating agar containing lysozyme (10μg/ml) incubated under anaerobic conditions for 5 days were also compared. None of the additives either individually or in combination in TPGY broth improved recovery of injured spore enumeration compared to processed controls without additives. Addition of lysozyme at concentrations of 5 and 10 μg/ml in TPGY broth improved initial recovery of injured spores of 17-B during the first 4 days of incubation but did not result in additional recovery at the end of the 4 month incubation compared to the processed control without lysozyme. Adding lysozyme at a concentration of 10 μg/ml to PYGS plating agar resulted in no effect on the recovery of high pressure and heat-injured 62-A and 17-B spores. The recovery counts of high pressure and heat-injured spores of 62-A and 17-B were lower (i.e., <1.0 log units) with PYGS plating agar compared to the MPN method using TPGY broth as the growth medium.
机译：添加剂和培养后处理条件对肉毒梭菌62-A（蛋白水解A型）菌株高压和热损伤（即在620 MPa和95和100℃下处理5分钟）孢子的回收率的影响研究了17-B（非蛋白水解性B型）。将高压和热损伤的孢子接种到含有各种添加剂（溶菌酶，L-丙氨酸，L-天冬氨酸，二吡啶甲酸，碳酸氢钠和乳酸钠）的TPGY（胰蛋白酶-蛋白ept-葡萄糖-酵母提取物）厌氧肉汤培养基中。浓度（0-10μg/ ml）单独或组合使用。从孵育4个月的含有溶菌酶（10μg/ ml）的TPGY肉汤中回收的高压和热损伤的62-A和17-B的孢子计数与从蛋白one-酵母提取物-葡萄糖-淀粉（PYGS）平板琼脂中回收的孢子计数还比较了将含有溶菌酶（10μg/ ml）的混合物在厌氧条件下孵育5天。与没有添加剂的加工对照相比，单独或在TPGY肉汤中组合使用的任何添加剂都不能提高受伤孢子计数的恢复率。在TPGY肉汤中添加浓度为5和10μg/ ml的溶菌酶可在培养的前4天提高受损17-B孢子的初始回收率，但与培养4个月后相比，并没有导致回收率进一步提高。没有溶菌酶的加工对照。向PYGS平板琼脂中添加浓度为10μg/ ml的溶菌酶对高压和热损伤的62-A和17-B孢子的回收没有影响。与使用TPGY肉汤作为生长培养基的MPN方法相比，使用PYGS平板琼脂的62-A和17-B高压和热损伤孢子的回收率更低（即<1.0 log单位）。

著录项

  • 来源
    《Food microbiology》 |2010年第5期|613-617|共5页
  • 作者

    N. R. Reddy; R. C. Tetzloff; G. E. Skinner;

  • 作者单位

    National Center for Food Safety and Technology, U.S. Food and Drug Administration, 6502 S. Archer Road, Summit-Argo, IL 60501, USA;

    National Center for Food Safety and Technology, Illinois Institute of Technology, 6502 S. Archer Road, Summit-Argo, IL 60501, USA Hormel Foods, 2 Hormel Place, Austin, MN 55912, USA;

    National Center for Food Safety and Technology, U.S. Food and Drug Administration, 6502 S. Archer Road, Summit-Argo, IL 60501, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    clostridium botulinum; high pressure processing; recovery of spores;

    机译：肉毒梭菌;高压处理;孢子的恢复;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号