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首页> 外文期刊>Food microbiology >Isolation, Identification And Typification Of Alicyclobacillus Acidoterrestris And Alicyclobacillus Acidocaldarius Strains From Orchard Soil And The Fruit Processing Environment In South Africa
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Isolation, Identification And Typification Of Alicyclobacillus Acidoterrestris And Alicyclobacillus Acidocaldarius Strains From Orchard Soil And The Fruit Processing Environment In South Africa

机译：南非果园土壤和水果加工环境中嗜酸拟杆菌和嗜酸拟杆菌的分离，鉴定和分类

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摘要

Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius are thermo-acidophilic, non-pathogenic, spore-forming bacteria that can survive the typical heat processing of fruit juices and concentrates. Bacterial endospores then germinate, grow and cause spoilage of acid food products. Species of Alicyclobacillus were isolated from orchard soil and a fruit concentrate production factory in South Africa. Preliminary identification of the isolates was based on morphological, biochemical and physiological properties. Identification at species level was done by PCR amplification using genus-specific primers and 16S ribosomal RNA (rRNA) gene sequencing. The majority of isolates belonged to the species A. acidoterrestris, but A. acidocaldarius was also isolated and identified. As far as we could determine, this is the first report of the isolation of A. acidoterrestris from wash water and soil outside a fruit processing plant, as well as the isolation of A acidocaldarius from vinigar flies. The genotypic relatedness between strains of A. acidoterrestris and between strains of A acidocaldarius was determined by RAPD-PCR. Sixteen isolates identified as A. acidoterrestris grouped into four clusters based on RAPD-PCR banding patterns, suggesting that they belong to at least four genotypic groups. Three isolateT:/PGN/ELSEVIER/YFMIC/web/ 00001155/s identified as A acidocaldarius gave three unique banding patterns.
机译：酸热脂环酸杆菌和酸热脂环酸杆菌是嗜热的，非致病性的，可形成孢子的细菌，可在果汁和浓缩物的典型热处理过程中存活下来。细菌的内生孢子随后发芽，生长并引起酸性食品的腐败变质。从果园土壤和南非的一家浓缩水果生产工厂中分离出脂环芽孢杆菌。分离物的初步鉴定是基于形态，生化和生理特性。使用属特异性引物和16S核糖体RNA（rRNA）基因测序，通过PCR扩增在物种水平上进行鉴定。多数分离物属于嗜酸曲霉种，但也分离并鉴定了嗜酸曲霉。据我们所能确定的，这是首次从水果加工厂外的洗涤水和土壤中分离嗜酸曲霉，以及从醋栗果蝇中分离嗜酸曲霉。通过RAPD-PCR确定嗜酸曲霉菌株之间和嗜酸曲霉菌株之间的基因型相关性。根据RAPD-PCR谱带划分模式，被鉴定为嗜酸曲霉的16种分离菌分为四个簇，表明它们至少属于四个基因型组。鉴定为酸性嗜酸菌的三个分离株T：/ PGN / ELSEVIER / YFMIC / web / 00001155 / s给出了三个独特的条带模式。

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