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首页> 外文期刊>Food microbiology >Isolation And Identification Of Spoilage Microorganisms Using Food-based Media Combined With Rdna Sequencing: Ranch Dressing As A Model Food
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Isolation And Identification Of Spoilage Microorganisms Using Food-based Media Combined With Rdna Sequencing: Ranch Dressing As A Model Food

机译：使用基于食物的培养基结合Rdna测序分离和鉴定腐败微生物：以牧场调味料为模型食品

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Investigating microbial spoilage of food is hampered by the lack of suitable growth media and protocols to characterize the causative agents. Microbial spoilage of salad dressing is sporadic and relatively unpredictable, thus processors struggle to develop strategies to minimize or prevent spoilage of this product. The objectives of this study were to (i) induce and characterize spoilage events in ranch-style dressing as a model food, and (ii) isolate and identify the causative microorganisms using traditional and food-based media, coupled with rDNA sequence analysis. Ranch dressing (pH 4.4) was prepared and stored at 25 ℃ for 14 d and microbial populations were recovered on MRS agar and ranch dressing agar (RDA), a newly formulated food-based medium. When isolates suspected as the spoilage agents were inoculated into ranch dressing and held at 25 ℃ for 9-10 d, three unique spoilage events were characterized. Using rDNA sequence comparisons, spoilage organisms were identified as Lactobacillus brevis, Pediococcus acidilactici, and Torulaspora delbrueckii. P. acidilactici produced flat-sour spoilage, whereas Lb. brevis resulted in product acidification and moderate gas production. The RDA medium allowed for optimum recovery of the excessive gas-producing spoilage yeast, T. delbrueckii. The isolation and identification strategy utilized in this work should assist in the characterization of spoilage organisms in other food systems.
机译：缺乏合适的生长介质和表征病原菌的方法阻碍了调查食物微生物变质。色拉调味品的微生物变质是偶发性的且相对不可预测，因此加工人员难以制定出最小化或防止该产品变质的策略。这项研究的目的是（i）在牧场式敷料中作为模型食品诱导和表征腐败事件，以及（ii）使用传统的和基于食物的培养基以及rDNA序列分析分离和鉴定致病微生物。制备牧场调味料（pH 4.4）并在25℃下保存14天，并在MRS琼脂和牧场配制琼脂（RDA）（一种新配制的食品基培养基）上回收微生物种群。当将怀疑是变质剂的分离株接种到牧场敷料中并在25℃下保温9-10 d时，表征了三个独特的变质事件。使用rDNA序列比较，腐败菌被鉴定为短乳杆菌，酸乳酸杆菌球菌和德氏螺旋藻。酸乳酸体育产生扁平酸变质，而磅。 brevis导致产品酸化并产生适度的气体。 RDA培养基可以最佳地回收过量产气的腐败酵母T. delbrueckii。这项工作中采用的隔离和识别策略应有助于表征其他食品系统中的腐败生物。

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