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首页> 外文期刊>Food microbiology >Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece
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Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece

机译：从希腊生牛，山羊精和羊乳中分离出的大肠杆菌O157的发生，致病基因和抗生素抗性

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摘要

The examination of 2005 raw bovine (n = 950), caprine {n = 460) and ovine (n = 595) bulk milk samples collected throughout several regions in Greece for the presence of Escherichia coli serogroup O157 resulted in the isolation of 29 strains (1.4%) of which 21 were isolated from bovine (2.2%), 3 from caprine (0.7%) and 5 from ovine (0.8%) milk. Out of the 29 E. coli 0157 isolates, only 12 (41.4%) could be classified as Shiga-toxigenic based on immunoassay and PCR results. All 12 Shiga-toxigenic E. coli serogroup 0157 isolates belonged to the E. coli O157:H7 serotype. All except one of the 12 Shiga-toxin positive isolates were stX_2-positive, five of which were also stx_1-positive. The remaining isolate was positive only for the stx_1 gene. All stx-positive isolates (whether positive for stx_1, stX_2 or stx_1 and stX_2) were also PCR-positive for the eae and ehxA genes. The remaining 17 E. coli 0157 isolates (58.6%) were negative for the presence of the H7 flagellar gene by PCR, tested negative for Shiga-toxin production both by immunoassay and PCR, and among these, only four and three strains were PCR-positive for the eae and ehxA genes, respectively. All 29 E. coli 0157 isolates displayed resistance to a wide range of antimicrobials, with the stx-positive isolates being, on average, resistant to a higher number of antibiotics than those which were stx-negative.
机译：对希腊多个地区收集的2005年生牛（n = 950），山羊（n = 460）和绵羊（n = 595）散装牛奶样本进行的检查，结果分离出O157大肠杆菌血清群，从而分离出29株（ 1.4％），其中21种是从牛（2.2％）中分离出来的，3种是从己内酰胺（0.7％）中分离的，5种是从羊（0.8％）牛奶中分离的。根据免疫测定和PCR结果，在29株大肠杆菌0157菌株中，只有12株（41.4％）可以归为志贺毒素。所有12株产志贺毒素的大肠杆菌血清群0157分离株均属于大肠杆菌O157：H7血清型。除12个志贺毒素阳性分离株中的1个外，所有分离株均为stX_2阳性，其中5个也为stx_1阳性。其余分离株仅对stx_1基因呈阳性。所有stx阳性分离株（对于stx_1，stX_2或stx_1和stX_2均为阳性）也对eae和ehxA基因呈PCR阳性。其余17株大肠杆菌0157分离株（58.6％）通过PCR检测是否存在H7鞭毛基因，通过免疫测定和PCR检测志贺毒素产生阴性，其中只有4和3株为PCR-分别对eae和ehxA基因呈阳性。所有29株大肠杆菌0157分离株均表现出对多种抗菌素的抗性，而stx阳性分离株对stx阴性的平均抗药性更高。

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  • 来源
    《Food microbiology》 |2009年第8期|865-871|共7页
  • 作者

    Nikolaos Solomakos; Alexandras Govaris; Apostolos S. Angelidis; Spyros Pournaras; Angeliki Rothi Burriel; Spyridon K. Kritas; Demetrios K. Papageorgiou;

  • 作者单位

    Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, Greece;

    Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, Greece;

    Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;

    Department of Microbiology, Medical School, University of Thessaly, 41110 Larissa, Greece;

    Laboratory of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, Greece;

    Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;

    Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    escherichia coli O157; STEC; antibiotic resistance; raw milk;

    机译：大肠杆菌O157;STEC;抗生素耐药性;生乳;

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