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首页> 外文期刊>Food microbiology >Fumonisin production in rice cultures of Fusarium verticillioides under different incubation conditions using an optimized analytical method
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Fumonisin production in rice cultures of Fusarium verticillioides under different incubation conditions using an optimized analytical method

机译：优化分析方法在不同培养条件下黄萎病水稻培养物中伏马菌素的生产

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摘要

Fumonisin B_1 (FB_1) and fumonisin B_2 (FB_2) are the main members of a family of mycotoxins produced by various fungal species belonging to the Gibberella fujikuroi complex. The present work shows the results of a comparative study of various clean-up and derivatization procedures for analysis of fumonisins in rice cultures. Fumonisins were extracted from rice with acetonitrile/water (50/50, v/v). For clean-up, three solid-phase extraction procedures were assayed (C_(18) cartridge, SAX cartridge, and a combination of both). Two reagents (o-phthaldialdehyde and 4-fluoro-7-nitro-benzofurazan) were studied comparatively for formation of fluorescent derivatives. The separation was carried out by LC using a fluorescence detector. The best procedure for analysis of fumonisins in rice involved clean-up with C_(18) cartridge and derivatization with o-phthaldialdehyde. The limit of detection was 0.010 mg kg~(-1) for both toxins. In the 10-500 mg kg~(-1) spiking level range, the recovery rates for FB_1 and FB_2 in rice varied from 94.6% to 103.6% and from 96.3% to 101.9%, respectively. The optimized analytical method for determination of fumonisins in rice was applied to the study of FB_1 and FB_2 production by four isolates of the G. fujikuroi species complex in rice cultures carried out at different temperatures and water activities to establish the influence of strain and environmental conditions on fumonisin production in this cereal. In general, fumonisin production was the highest at 20℃ and lowest at 37℃. Four of the five assayed water activity (a_w) values (0.97, 0.98, 0.99, and 1.0) did not affect significantly fumonisin accumulation but fumonisins were not detected in cultures when a_w was 0.96.
机译：伏马菌素B_1（FB_1）和伏马菌素B_2（FB_2）是由富士赤霉菌复合体的各种真菌产生的真菌毒素家族的主要成员。本工作显示了对用于水稻培养物中伏马毒素的各种净化和衍生化程序进行比较研究的结果。从大米中用乙腈/水（50/50，v / v）提取伏马毒素。为了进行净化，分析了三种固相萃取程序（C_（18）柱，SAX柱以及两者的组合）。对两种试剂（邻苯二甲醛和4-氟-7-硝基-苯并呋喃山）进行了研究，以形成荧光衍生物。使用荧光检测器通过LC进行分离。分析大米中烟曲霉毒素的最佳方法是用C_（18）柱进行净化并用邻苯二甲醛衍生化。两种毒素的检出限均为0.010 mg kg〜（-1）。在10-500 mg kg〜（-1）加标水平范围内，水稻中FB_1和FB_2的回收率分别为94.6％至103.6％和96.3％至101.9％。测定水稻中烟曲霉毒素的最佳分析方法被用于研究在不同温度和水分活动下进行的水稻培养中的四种藤霉菌复合体菌株FB_1和FB_2的产生，以建立菌株和环境条件的影响。谷物中伏马毒素的含量。通常，伏马菌素的产量在20℃时最高，而在37℃时最低。五个测定的水活度（a_w）值中的四个（0.97、0.98、0.99和1.0）不会显着影响伏马菌素的积累，但是当a_w为0.96时在培养物中未检测到伏马菌素。

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