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首页> 外文期刊>Food microbiology >Development of a real-time PCR assay targeting the sporulation gene, spo0A, for the enumeration of thermophilic bacilli in milk powder
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Development of a real-time PCR assay targeting the sporulation gene, spo0A, for the enumeration of thermophilic bacilli in milk powder

机译：开发针对孢子形成基因spo0A的实时PCR测定法，用于乳粉中嗜热杆菌的计数

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摘要

Thermophilic bacilli, such as Anoxybacillus, Geobacillus and Bacillus, are common contaminants growing within the processing lines of milk powder producing factories. These contaminants are used as indicator organisms for plant hygiene and specification limits based on their numbers have been implemented to ensure milk powder quality. In this study, we present a SYBR Green-based real-time PCR assay for the rapid detection and enumeration of these thermophilic bacilli in milk powder using the spo0A sporulation gene as quantification target. With this method the detection of thermophilic bacilli in milk powder can be accomplished within 1 h. The detection limit for reconstituted and inoculated milk was 80 vegetative cfu ml~(-1) and 640 spores ml~(-1), respectively.
机译：嗜热杆菌，如脱氧芽孢杆菌，地芽孢杆菌和芽孢杆菌，是奶粉生产工厂生产线中常见的污染物。这些污染物被用作指示植物卫生的指示生物，并且已根据其数量实施了规格限制以确保奶粉质量。在这项研究中，我们提出了一种基于SYBR Green的实时PCR检测方法，以spo0A孢子形成基因为定量目标，可以快速检测和枚举奶粉中的嗜热杆菌。用这种方法可以在1小时内完成奶粉中嗜热杆菌的检测。重构牛奶和接种牛奶的检出限分别为80营养cfu ml〜（-1）和640孢子ml〜（-1）。

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