• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Comparison of four methods to enumerate probiotic bifidobacteria in a fermented food product
【24h】

Comparison of four methods to enumerate probiotic bifidobacteria in a fermented food product

机译：四种计数发酵食品中益生菌双歧杆菌的方法的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Four methods of enumeration were compared by monitoring levels of probiotic bifidobacteria in fermented oat drink during storage. Strains of Bifidobacterium longum and B. lactis were quantified by plate counts, fluorescent in situ hybridization (FISH), quantitative real-time PCR and commercial LIVE/DEAD~® BacLight™ bacterial viability kit, and the methods were further developed to suit the enumeration of bifidobacteria in fermented foods. Plate counts of both B. lactis and B. longum were lower than the PCR and FISH counts. The LIVE/DEAD counts of B. lactis were comparable to PCR and FISH counts. The plate counts of B. lactis were slightly but significantly lower than LIVE/DEAD counts, suggesting that the cells that were not able to grow on plates may have become dormant. The plate counts of B. longum were several log units lower than LIVE/DEAD counts, suggesting that a remarkable part of the cells were dormant. Real-time PCR and FISH were shown to suit the quantification of the total amount of probiotic bifidobacteria in foods. Plate counts and LIVE/DEAD counts provided conflicting information on viability especially in the case of B. longum. We conclude that the choice of enumeration method for probiotic bacteria may have significant effect on the results of the analysis. The strain-specific properties and the objects of the analysis should be taken into account when enumeration methods for different probiotic strains are chosen.
机译：比较了四种计数方法，通过监测燕麦储存期间发酵燕麦饮料中益生菌双歧杆菌的含量。通过平板计数，荧光原位杂交（FISH），实时荧光定量PCR和商业LIVE / DEAD〜®BacLight™细菌生存力试剂盒对长双歧杆菌和乳酸双歧杆菌的菌株进行定量，并对方法进行进一步开发以适合枚举食品中双歧杆菌的数量。乳酸双歧杆菌和长双歧杆菌的平板计数均低于PCR和FISH计数。乳酸双歧杆菌的LIVE / DEAD计数与PCR和FISH计数相当。乳酸双歧杆菌的平板计数比LIVE / DEAD计数略低但显着较低，这表明不能在平板上生长的细胞可能已经处于休眠状态。长双歧杆菌的平板计数比LIVE / DEAD计数低几个对数单位，表明该细胞的显着部分处于休眠状态。实时荧光定量PCR和FISH被证明适合定量食品中益生菌双歧杆菌的总量。板计数和活/死计数提供了关于生存能力的相互矛盾的信息，特别是在长双歧杆菌的情况下。我们得出结论，选择益生菌的计数方法可能对分析结果产生重大影响。选择不同益生菌菌株的计数方法时，应考虑菌株的特异性和分析对象。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号