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首页> 外文期刊>Food microbiology >Identification of the gene encoding a putative tyrosine decarboxylase of Carnobacterium divergens 508. Development of molecular tools for the detection of tyramine-producing bacteria
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Identification of the gene encoding a putative tyrosine decarboxylase of Carnobacterium divergens 508. Development of molecular tools for the detection of tyramine-producing bacteria

机译：鉴定编码假单胞菌508的酪氨酸脱羧酶的基因。开发用于检测产生酪氨酸的细菌的分子工具

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摘要

The gene encoding a putative tyrosine decarboxylase (TyrDC) in Carnobacterium divergens 508 was identified by multiple steps of inverse and conventional PCR. The coding region of the tyrdc gene was 1875 bp in size. A putative tyrosyl-tRNA synthetase gene was identified from a partial gene sequence upstream of the tyrdc gene and another partial ORF potentially coding for an amino acid transporter was identified downstream. Comparison to the TyrDC operon of Enterococcus faecalis showed that these three genes are probably part of an operon involved in tyramine production in C. divergens 508. Homology search allowed for the design of a set of consensual primers in the tyrdc gene. These primers were used in PCR experiments and allowed for the detection of tyramine producing bacteria. In the future, these primers could be used for the early detection of potentially tyramine-forming bacteria in food and beverages.
机译：通过反向和常规PCR的多个步骤鉴定了在食肉克氏杆菌508中编码假定的酪氨酸脱羧酶（TyrDC）的基因。 tyrdc基因的编码区大小为1875 bp。从tyrdc基因上游的部分基因序列中鉴定出一个推定的酪氨酰-tRNA合成酶基因，而在下游鉴定出另一种可能编码氨基酸转运蛋白的部分ORF。与粪肠球菌的TyrDC操纵子的比较表明，这三个基因可能是参与C. divergens 508中酪胺生产的操纵子的一部分。同源性搜索允许设计tyrdc基因中的一组共有引物。这些引物用于PCR实验，可用于检测产生酪胺的细菌。将来，这些引物可用于早期检测食品和饮料中可能形成酪胺的细菌。

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