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A medium for the presumptive detection of Enterobacter sakazakii in infant formula

机译：推定婴儿配方食品中阪崎肠杆菌的培养基

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A differential-elective medium for the isolation of Enterobacter sakazakii was developed. It is based on the presence of an enzyme α-glucosidase in this species that can differentiate this organism from other members of the family Enterobacteriaceae. Nutrient agar (NA) was supplemented with 4-methyl-umbelliferyl α-D-glucoside (α-MUG). This substrate was metabolized by the enzyme to yield an UV fluorescent product. E. sakazakii formed yellow pigmented UV fluorescent colonies on NA with α-MUG. Fifty-six E. sakazakii strains from culture collections were examined and found positive for both characteristics. Fifty-eight formula products from 11 countries were sampled in their entity by using a pre- and selective enrichment method following ISO 8523. The selective enrichment was streaked on NA + α-MUG and violet red brilliant green (VRBG) agar. Twenty-one (36%) of products were positive for Enterobacteriaceae. E. sakazakii was isolated from eight out of 58 infant formula products representing 13.8%.
机译：开发了一种用于分离阪崎肠杆菌的差异选择培养基。这是因为该物种中存在一种酶α-葡萄糖苷酶，该酶可以使该生物体与肠杆菌科的其他成员区分开。营养琼脂（NA）补充有4-甲基伞形糖基α-D-葡萄糖苷（α-MUG）。该底物被酶代谢，产生紫外线荧光产物。阪崎肠杆菌与α-MUG在NA上形成了黄色的UV荧光菌落。检查了来自培养物收集物中的56种阪崎肠杆菌菌株，发现这两个特征均为阳性。通过遵循ISO 8523的预富集和选择性富集方法，在其实体中对来自11个国家的58个配方产品进行了采样。选择性富集在NA +α-MUG和紫红色亮绿色（VRBG）琼脂上划线。 21种产品（36％）对肠杆菌科呈阳性反应。阪崎肠杆菌是从58种婴儿配方食品中的8种中分离出来的，占13.8％。

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