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Densitometric determination of carbohydrates: Application to purification and molecular weight determination of polysaccharide from Hericium erinaceum mushroom

机译:碳水化合物的光密度测定:在猴头菇蘑菇多糖的纯化和分子量测定中的应用

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摘要

A simple, precise, sensitive and quick densitometric method for quantitative total carbohydrate determination as well as for preparation of elution profiles of polysaccharides was developed and validated. The method is based on spotting 3 μL of sample solution by use of disposable micro capillary pipettes as a single drop on a TLC silica gel plate. The plate is then submitted to in situ derivatization with orcinol-sul-furic acid reagent and is scanned densitometrically at 560 nm (for hexoses, deoxy sugars, and uronic acids) or at 610 nm (for pentoses and ketoses). It was demonstrated that the densitometric method has a greater linearity range and similar precision in comparison to the widely used colorimetric phe-nol-sulfuric acid method. The proposed method was successfully applied to elution profile preparation during purification and molecular weight determination of a novel polysaccharide isolated from the mycelium of edible and medicinal mushroom Hericium erinaceum.
机译:开发并验证了一种简单,精确,灵敏和快速的光密度测定法,用于定量总碳水化合物的测定以及多糖洗脱谱的制备。该方法基于通过使用一次性微型毛细管移液器在TLC硅胶板上滴一滴液来点样3μL样品溶液。然后,将该板用蓖麻酚-磺基-呋喃酸试剂原位衍生,并在560 nm(对于己糖,脱氧糖和糖醛酸)或610 nm(对于戊糖和酮糖)进行光密度扫描。结果表明,与广泛使用的比色苯酚-酚-硫酸法相比,光密度法具有更大的线性范围和相似的精密度。该方法已成功地应用于从食用和药用蘑菇猴头菌丝体菌丝体中分离得到的新型多糖的纯化和分子量测定中的洗脱曲线制备。

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