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首页> 外文期刊>Food Science and Biotechnology >Induction of apoptosis in human hepatoma SMMC-7721 cells using 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone, a chalcone from buds of Cleistocalyx operculatus
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Induction of apoptosis in human hepatoma SMMC-7721 cells using 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone, a chalcone from buds of Cleistocalyx operculatus

机译：使用2'，4'-二羟基-6'-甲氧基-3'，5'-二甲基查耳酮（一种来自盲le的芽中的查尔酮）诱导人肝癌SMMC-7721细胞凋亡

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摘要

The molecular mechanism to induce apoptosis in human hepatoma SMMC-7721 cells was investigated using 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) isolated from the buds of Cleistocalyx operculatus. Typical morphological changes in apoptotic body formation after DMC treatment were observed using acridine orange (AO)/ethidium bromide (EB) staining. After 48 h of incubation with 0, 6.25, 12.5, and 25 μM of DMC, the percentages of annexin V-FITC-positive/PI negative cells were 1.51, 9.33, 33.84, and 61.32%, respectively. Apoptosis induced by DMC was blocked by z-VAD-fmk, a caspase inhibitor. Analysis of fluorescence intensities of rhodamine 123 in cells showed that DMC induced a loss of mitochondrial membrane potential (ΔΨm). Western blot results showed that DMC decreased the Bcl-2/Bax ratio, suggesting that the Bcl-2 family is involved in control of apoptosis. All of these signal transduction pathways are involved in initiating apoptosis. DMC is a potential anticancer agent that deserves further exploration.
机译：利用从oper骨花芽中分离出的2'，4'-二羟基-6'-甲氧基-3'，5'-二甲基查尔酮（DMC）研究了诱导人肝癌SMMC-7721细胞凋亡的分子机制。使用a啶橙（AO）/溴化乙锭（EB）染色观察到DMC处理后凋亡小体形成的典型形态学变化。与0、6.25、12.5和25μMDMC孵育48小时后，膜联蛋白V-FITC阳性/ PI阴性细胞的百分比分别为1.51、9.33、33.84和61.32％。 DMC诱导的凋亡被caspase抑制剂z-VAD-fmk阻断。细胞中若丹明123的荧光强度分析表明，DMC引起线粒体膜电位（ΔΨm）的损失。蛋白质印迹结果表明DMC降低了Bcl-2 / Bax比值，表明Bcl-2家族参与细胞凋亡的控制。所有这些信号转导途径都参与启动细胞凋亡。 DMC是潜在的抗癌药物，值得进一步探索。

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  • 来源
    《Food Science and Biotechnology》 |2013年第5期|1-7|共7页
  • 作者

    Chun-Lin Ye; Xuan-Gan Liu; Qi Huang; Xian-Liang Zhao;

  • 作者单位

    School of Biological and Chemical Engineering Zhejiang University of Science and Technology">(1);

    School of Biological and Chemical Engineering Zhejiang University of Science and Technology">(1);

    School of Biological and Chemical Engineering Zhejiang University of Science and Technology">(1);

    School of Biological and Chemical Engineering Zhejiang University of Science and Technology">(1);

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  • 正文语种 eng
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  • 关键词

    Cleistocalyx operculatus; flavonoid; apoptosis; caspase; mitochondria;

    机译：圆叶鞘;类黄酮细胞凋亡半胱天冬酶线粒体;

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