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Optimization of microwave-assisted extraction of bioactive alkaloid compounds from Rhizoma Coptidis (Coptis chinensis Franch.)

机译:微波辅助提取黄连生物活性生物碱化合物的工艺优化

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Microwave-assisted extraction (MAE) technique was employed and optimized for microwave power, irradiation time and solvent (ethanol) concentration using a central composite design and response surface methodology for the efficient extraction of bioactive alkaloid compounds from Rhizoma coptidis (Coptis chinensis Franch.). Alkaloid compounds were successfully isolated and quantified by high-performance liquid chromatography with ultraviolet detection. Maximum yields of alkaloid constitutes were predicted based on desirability option, and optimal conditions for MAE were microwave power of 180W, irradiation time of 5min, and ethanol concentration of 50%. Reliability of the method was confirmed by verification experiments performed under optimal conditions, and the experimental values (TAC of 333.94 mg BCE/g d.w., BC of 71.43 mg BCE/g d.w., and PC of 15.58 mg PCE/g d.w.) matched well with estimated values, suggesting that the estimated models were reliable and valid for MAE of alkaloids.
机译:采用微波辅助提取(MAE)技术并使用中央复合设计和响应面方法对微波功率,辐射时间和溶剂(乙醇)浓度进行了优化,以从黄连中有效提取生物活性生物碱化合物。 。通过高效液相色谱和紫外检测,成功分离并定量了生物碱化合物。基于合意性选项,预测了生物碱成分的最大产量,MAE的最佳条件是微波功率为180W,照射时间为5min,乙醇浓度为50%。通过在最佳条件下进行的验证实验确认了该方法的可靠性,并且实验值(TAC为333.94 mg BCE / g dw,BC为71.43 mg BCE / g dw和PC为15.58 mg PCE / g dw)与该值非常匹配估计值,表明估计的模型对于生物碱的MAE是可靠且有效的。

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